反相高效液相色谱法同时测定毛两面针中氯化两面针碱、乙氧基白屈菜红碱和毛两面针素的含量  

RP-HPLC determination of nitidine chloride,ethoxychelerythrine and toddaloactone in Zanthozylum nitidum(Roxb) DC.f. fastuosum How ex Huang

作　　者：孙培文[1,2] 刘海涛[1,2] 雷鹏[1,2] 李新中[1,2] 黄琪[1,2] 李观勇[3] 

机构地区：[1]中南大学湘雅医院药剂科,长沙410008 [2]中南大学药学院,长沙410013 [3]桂林医学院

出　　处：《中南药学》2011年第3期175-178,共4页Central South Pharmacy

基　　金：湖南省中医药科技基金重点课题(编号:2009008);长沙市科技局重点项目(编号:K0802115-31)

摘　　要：目的建立同时测定毛两面针中氯化两面针碱、乙氧基白屈菜红碱和毛两面针素的含量方法。方法采用RP-HPLC法,色谱柱：Promosil-C18柱（250 mm×4.6 mm,5μm）;流动相：乙腈-水（含0.2%磷酸和0.2%三乙胺）（28∶72）;检测波长：273、328 nm;流速：1.0 mL.min-1;柱温：30℃。结果氯化两面针碱、乙氧基白屈菜红碱和毛两面针素分别在0.011~0.139μg（r=0.999 7）、0.010~0.125μg（r=0.999 9）、0.102~1.23μg（r=0.999 9）与峰面积呈良好线性关系;加样回收率分别为95.8%、103.7%、99.3%,其RSD分别为2.6%、1.1%和2.9%。结论该方法简便、快速、准确,重复性好,可用于毛两面针中氯化两面针碱、乙氧基白屈菜红碱和毛两面针素含量的同时测定。Objective To establish a method for the determination of nitidine chloride,ethoxychelerythrine and toddaloactone in Zanthozylum nitidum（Roxb） DC.f.fastuosum How ex Huang simultaneously.Methods The RP-HPLC system consisted of Promosil C18 column（250 mm × 4.6 mm,5 μm） with the mobile phase of acetonitrile solution-water solution（containing 0.2% phosphate acid and 0.2% triethylamine）（28∶72）.The detection wave length was 273 nm,328 nm.The flow rate was 1.0 mL·min-1 and the column temperature was 30 ℃.Results The linear response ranges were 0.011-0.139 μg（r=0.999 7）,0.010-0.125 μg（r=0.999 9） and 0.102-1.23 μg（r=0.999 9）,respectively.The average recoveries（n=6）were 95.8%,103.7%,and 99.3%,respectively.Conclusion The method is quick,simple,and reproducible for the determination of nitidine chloride,ethoxychelerythrine and toddaloactone in Zanthozylum nitidum（Roxb） DC.f.fastuosum How ex Huang simultaneously.

关 键 词：毛两面针 氯化两面针碱 乙氧基白屈菜红碱 毛两面针素 反相高效液相色谱法 

分 类 号：R965[医药卫生—药理学]