高效液相色谱法测定灯盏细辛胶囊中焦袂康酸的含量  

Determination of pyromeconic acid in Dengzhan Xixin Jiaonang by HPLC

作　　者：龙海燕[1] 黄晓燕[1] 

机构地区：[1]湖南省药品检验所,长沙410001

出　　处：《中南药学》2011年第3期188-190,共3页Central South Pharmacy

基　　金：国家药典委员会国家药品标准提高行动计划课题(编号:561)

摘　　要：目的建立高效液相色谱法测定灯盏细辛胶囊中焦袂康酸含量的方法。方法采用高效液相色谱法,色谱柱：Agilent C18（TC）（250 mm×4.6 mm,5μm）;流动相：甲醇-0.1%磷酸溶液,梯度洗脱;流速：0.8 mL.min-1;检测波长：270 nm;柱温：35℃。结果焦袂康酸进样量在0.010 176~0.848μg与峰面积线性关系良好,r=0.999 0,平均加样回收率为103.3%,RSD=0.34%。结论所建立的含量测定方法简便可行,结果准确可靠,可用于灯盏细辛胶囊中焦袂康酸的含量测定。Objective To establish an HPLC method for the determination of pyromeconic acid in Dengzhan Xixin Jiaonang.Methods HPLC method was used with Agilent C18（TC） column（250 mm ×4.6 mm,5 μm）.The mobile phase consisted of methanol and 0.1% H3PO4 solution with gradient elution,and the flow rate was 0.8 mL·min-1.The detective wavelength was 270 nm,and the column temperature was 35 ℃.Results The linear range of pyromeconic acid was 0.010 176-0.848 μg.The average recovery of pyromeconic acid was 103.3% with RSD=0.34%.Conclusion The method is accurate and can be used for the content determination of pyromeconic acid in Dengzhan Xixin Jiaonang.

关 键 词：灯盏细辛胶囊 焦袂康酸 高效液相色谱法 

分 类 号：R927.1[医药卫生—药学]