反相高效液相色谱法同时测定藤三七不同部位中尿嘧啶和假鹰爪黄酮的含量  

Simultaneous determination of the content of uracil and desmosflavone in different parts of Boussingaultia gracilis Miers var. Pseudobaselloides Bailey by RP-HPLC

作　　者：刘佳[1] 李国萍[2] 慕善学[1] 毕开顺[3] 

机构地区：[1]沈阳药科大学高等职业技术学院,沈阳110026 [2]辽河石油勘探局妇婴医院,辽宁盘锦124010 [3]沈阳药科大学药学院,沈阳110016

出　　处：《中南药学》2011年第4期261-264,共4页Central South Pharmacy

摘　　要：目的建立测定藤三七不同部位(包括珠芽、茎、叶和花)中尿嘧啶和假鹰爪黄酮含量的反相高效液相色谱法。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),流动相:乙腈(A)-0.05%磷酸溶液(B),进行梯度洗脱,程序为:3%A(0～6 min),3%～60%A(6～8 min),60%～80%A(8～13 min),80%A(13～20 min);流速为1 mL.min-1;检测波长为254 nm;柱温:35℃。结果尿嘧啶在0.705～7.05μg.mL-1与峰面积呈良好线性关系,r=0.999 9(n=6),平均回收率(n=9)为99.2%(RSD=1.3%);假鹰爪黄酮在0.792～7.92μg.mL-1与峰面积呈良好线性关系r=0.999 9(n=6),平均回收率(n=9)为99.0%(RSD=1.2%)。结论该方法简单、准确,为藤三七药材质量评价提供了可靠依据。Objective To develop an RP-HPLC method for the determination of the content of uracil and desmosflavone in the bulbule,stem,leaf,flower of Boussingaultia gracilis Miers var.Pseudobaselloides Bailey.Methods Uracil and desmosflavone were isolated by silica gel column chromatography and identified by spectral data.A Kromasil C18 column（250 mm×4.6 mm,5 μm） with a solvent system consisting of acetonitrile（A）-0.05 % H3PO4（B）：3%A（0-6 min）;3%-60%A（6-8 min）;60%-80% A（8-13 min）;80 % A（13-20 min）was used,the flow rate was 1 mL·min-1,the detecting wavelength was 254 nm,and the column temperature was 35 ℃.Results There was good linearity between the peak area and the sample contents injected at 0.705-7.05 μg·mL-1,r=0.999 9（n=6） and 0.792-7.92 μg·mL-1,r=0.999 9（n =6） for uracil and desmosflavone respectively.The average recovery of uracil and desmosflavone was 99.2 %（RSD=1.3 %） and 99.0 %（RSD=1.2 %）,respectively.Conclusion The method is sensitive,accurate,rapid,and suitable for the quality control of Boussingaultia gracilis Miers var.Pseudobaselloides Bailey.

关 键 词：尿嘧啶 假鹰爪黄酮 藤三七 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R284.1