荷叶HPLC-UV指纹图谱研究  

Fingerprints of Folium Nelumbinis with HPLC-UV

作　　者：杨鹏[1] 陈波[2] 陈希平[1] 邓克国[2] 

机构地区：[1]湖南中医药大学药学院,长沙410208 [2]湖南师范大学化学生物学及中药分析教育部重点实验,长沙410081

出　　处：《中南药学》2011年第4期290-294,共5页Central South Pharmacy

基　　金：湖南省自然科学基金项目(编号:06JJ4039);湖南省科技厅条件支撑项目(编号:2007TP4020)

摘　　要：目的采用HPLC法建立荷叶药材的指纹图谱。方法 Ultimate XB-C18色谱柱(4.6 mm×250 mm,5μm),流动相为0.1%甲酸+0.2%三乙胺溶液(A)-乙腈(B)进行梯度洗脱,检测波长254 nm,体积流量1.0 mL.min-1,柱温35℃。结果建立了荷叶的HPLC-UV的指纹图谱,指定出11个共有指纹峰,共指认3个共有峰的结构并对不同产地和不同采收时期荷叶的相似度进行了比较。结论本方法操作简便,快速,重现性好,为荷叶的鉴别和质量控制提供有效依据。Objective To develop a chromatographic fingerprinting method for the quality evaluation of Folium Nelumbinis using HPLC-UV.Methods Ultimate XB-C18 column（4.6 mm × 250 mm,5 μm） was used with acetonitrile-water（0.1% formic acid and 0.2% triethylamine） as the mobile phase at 35 ℃.A well-designed gradient elution program was used.The UV wavelength was 254 nm,with the flow rate of 1 mL·min-1.Results High-quality chromatographic fingerprint of Folium Nelumbinis was obtained.A total of 11 common fingerprint peaks were detected and the structures of 3 were identified.Samples from different origins were compared and so were samples with different harvest time.Conclusion The method is characterized with simple operation procedure and good repeatability,indicating this method can be effectively used for the identification and quality control of Folium Nelumbinis.

关 键 词：荷叶 高效液相色谱 指纹图谱 芦丁 金丝桃苷 荷叶碱 

分 类 号：R284[医药卫生—中药学] O234[医药卫生—中医学]