高速逆流色谱法分离纯化白鲜皮中梣酮的初步研究  

Preliminary Study on Isolation and Purification of Fraxinellone from Dictamnus dasycarpus by High-speed Countercurrent Chromatography

作　　者：张红晶[1,2] 王琦[1] 时东方[2] 赵立春[3] 王晶[2] 吴桂梅[2] 刘春明[2] 

机构地区：[1]吉林农业大学,吉林长春130118 [2]长春师范学院,吉林长春130032 [3]河北大学药学院,河北保定071002

出　　处：《浙江林业科技》2010年第6期52-54,共3页Journal of Zhejiang Forestry Science and Technology

摘　　要：利用高速逆流色谱技术分离纯化白鲜皮中的梣酮,结果表明,选择V(正己烷):V(乙酸乙酯):V(乙醇):V(水)=1:1:1:1系统来分离,上相作为固定相,下相作为流动相,流速为1.5 mL/min,仪器转速1 000 r/min,分离结果经高效液相色谱(HPLC)检测纯度达到99%。High-speed Countercurrent Chromatography（HSCCC） was used to isolate and purify Fraxinellone from Dictamnus dasycarpus.Isolation was performed with a two-phase solvent system composed of hexane：ethylacetate：ethanol：water = 1：1：1：1：1（V/V）by eluting the lower aqueous phase at a flow-rate of 1.5 mL/min and a revolution speed of 1 000 r/min.The purified Fraxinellone could reach purity of 99% by high performance liquid chromatograph（HPLC）.HSCCC is recommended to prepare and purify the active constituents from D.dasycarpus.

关 键 词：白鲜皮 高速逆流色谱 分离 梣酮 

分 类 号：S567.2[农业科学—中草药栽培]