Effects of nutrient profiles and light regime on the production of exopolysaccharide by Porphyridium cruentum  

作　　者：SUN Li-qin WANG Chang-hai SHI Lei 

机构地区：[1]Marine Biochemical Engineering Lab, Dalian University of Technology, Liaoning 116024, China [2]Ocean School of Yamai University ,Yantai 264005, China

出　　处：《Journal of Environmental Science and Engineering》2008年第10期71-79,共9页环境科学与工程（英文版）

摘　　要：In order to increase the yield of polysaccharides from Porphyridium cruentum and the cell biomass, we investigated several important cultural conditions such as nutrient-salts, light irradiance as well as growth conditions in the flat plate photobioreacturs （FPPBR） with different light-path. We have found that the optimal （OCM II ） was a superior medium for extracellular polysaccharide production than the f/2 and Koch medium, and the polysaccharide production with OCM II was increased by 1.79 times and 1.62 times compared with that of f/2 and Koch medium; the yields of polysaccharides were also improved by optimizing the light intensity and irradiation length Furthermore, we significantly enhanced the yields of both the biomass and extracellular polysaccharidc using a small light-path photobioreactor, and about 2.1 times increase of polysaccharide production was observed in FPPBR-30 than in FPPBR-100. Overall, the process for polysaccharides production was improved and this would facilitate further studies on such polysaccharidcs such as their antiviral and antitumor activities.

关 键 词：cell growth culture condition extracellular polysaccharide fiat-plate photobioreactor Porphyridium cruentum 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ929[轻工技术与工程—食品科学与工程]