Primary study on anther culture of balsum pear for callus and organ formation  

作　　者：LI Huan-xiu CHEN Jia ZHENG Yang-xia YAN Ze-sheng HE Yan 

机构地区：[1]Department of Horticulture, College of Forestry and Horticulture, Sichuan Agriculture University, Ya＇an Sichuan 625014, China

出　　处：《Journal of Life Sciences》2008年第1期12-18,共7页生命科学（英文版）

基　　金：The authors would like to thank Dr. WANG Xiao-rong and DENG Qun-xian for their help in the tests. This study was supported by the Education Bureau Science Foundation of Sichuan Province （No. 2005A003） and the Sichuan Agricultural University Science Foundation （No. 3305）.

摘　　要：The balsum pear （Momordica charantia L. ） anthers in the monokaryotic stage of microspore development were cultured in this experiment. Different Plant growth regulators＇ combinations, base media and carbon sources were studied for callus formation and organ differentiation from balsum pear anthers. The result showed that the best media for callus inducement was： MS＋BA 0.5 mg/L＋NAA 0.2 mg/L＋ 2, 4-D 0.5 mg/L＋KT 2.0 mg/L, with 3% sugar and 0.8% agar. The best media to induce roots from balsum pear anther callus was： MS＋NAA 0.05 rag/L＋ KT 0.5 rag/L, with 3% sugar and 0.8% agar. Most of adventitious roots from callus were triploid（2N=3X=33）

关 键 词：balsum pear （Momordica charantia L. ） antherculture callus inducement organ differentiation 

分 类 号：S668.403.5[农业科学—果树学] S685.23[农业科学—园艺学]