轉玉米PEPC基因水稻開花期PEPC酶及碳代謝酶活性的變化  

Changes of PEPC carboxylase and other carbon metabolism enzyme activity in PEPC transgenic rice during flowering

作　　者：李霞[1] 

机构地区：[1]江蘇省農業科學院糧食作物研究所,南京210014

出　　处：《Journal of Life Sciences》2008年第10期11-20,37,共11页生命科学（英文版）

基　　金：本研究得到國家自然科學基金項目（項目編號：30871459）,江蘇省自然科學基金項目（項目編號：BK2004429）,江蘇省農業科學院基項目（項目編號：6510707,6110704）資助.

摘　　要：爲阐明轉PEPC基因水稻增産的生理基礎，用第八代轉PEPC基因水稻（PC）和未轉基因原種野生型（WT）爲供试材料，分别测定了稻株開花期地上部分斡物贾的累積、籽粒中可溶性糖、激粉和蛋白質含量以及其蔗糖磷酸合成酶（SPS）、蔗糖合成酶（SS）、ADPG焦磷酸化酶、澱粉合成酶、殿粉分支酶、硝酸還原酶，谷氨酰胺合成酶（GS）和谷草轉氨酶（GOT）活性的變化。结果表明：轉PEPC基因水稻籽粒中可溶性糖含量比未轉基因水稻顯著增加，而轉PEPC基因水稻籽粒中增加的可溶性糖較多地蒋化爲其籽粒中的蛋白質，其生理基礎舆SPS，SS以及籽粒中ADPG焦磷酸化酶活性顯颗著提高有關。舆此同時，轉基因植物菜片中氮代谢相關的硝酸還原酶（NR），谷氨酰胺合成酶（GS）和谷草轉氨酶（GOT）活性也提高，也有利于增加其開花後期籽粒中蛋白質的合成。To elucidate the photosynthetic physiological characteristics for enhancing yield of phosphoenolpyruvate carboxylase transgenic rice line; using the PEPC transgenic rice and untransformed wide type rice as materials, dry matter accumulation, contents of soluble sugar and starch as well as protein in the aerial part during flowering stages was determined in these rice plant. Furthermore, the activities of surocse-p-synthase（SPS）, sucrose synthase （SS）, ADP glucose pyriphosphorylase （ADPGPase）, Starch synthase （SSase） and Q-enzyme in the grain, nitrate reductase （NR）, Glutamic-oxalacetic transaminase （GOT） and Glutamine synthetase （GS） during flowering stage were measured in PEPC transgenic rice lines and untransformed wild type rice： Relative to untransformed wild type rice, dry matter weights in the leaf, stem and sheath as well as panicle of PEPC transgenic rice were increase, with most in panicle. 15th after flowering, soluble sugar content and protein content in the grains of PEPC transgenic rice were raised significantly, with no changes in starch content. It is interesting that enzymes such as Nitrate reductase （NR）, Glutamic-oxalacetic transaminase （GOT） and Glutamine synthetase （GS） in leaves of PEPC transgenic rice plant were also increased, which were related with the changes of protein content in the grain of trangenic plants. It implied that the increase of protein of grain in PEPC transgenic plant is related to the effect of introduction of PEPC gene.

关 键 词：轉PEPC基因水稻 幹物質累積 碳代謝 氮代謝 

分 类 号：Q[生物学]