基于生物衍生骨的组織工程骨的髓外構建和體内移植  

In vitro fabrication of tissue-engineered bones based on the bio-derived scaffolds and in vivo transplantation

作　　者：宋克東 劉天廣 馬學虎 崔占峰 

机构地区：[1]大連理工大學斡细胞舆组織工程研究室,大連116024 [2]牛津大學工程科學系,牛津OXI3P

出　　处：《Journal of Life Sciences》2008年第10期45-50,共6页生命科学（英文版）

摘　　要：本研究使用生物衍生骨作爲支架材料在旋轉壁式生物反應器中進行组織工程骨的構建，并使用構建出的组織進行新西蘭兔橈骨缺损的修復。将成骨细胞經绿色荧光蛋白轉染後，以1×10^6ells／ml接種到衍生骨支架材料上在反應器中構建一周。将以同種细胞密度接種的成骨细胞_支架材料的構建物放置于培養瓶中培養作爲對照组。構建出的復合物的形態和结構通過倒置顯微镜、掃描電镜、HE染色和甲苯胺蘭染色等方式進行检测。此後，使用上述在反應器中構建出的工程化骨组織進行新西蘭兔橈骨缺损的修復。结果顯示在反應器中培養的细胞擴增数量是静態構建的5倍，并且在反應器中構建的骨组織生長良好。移植8周後試验组顯示出最好的修復效果。由上述结果可知，通過反應器内部流體對流所座生的應力刺激，可提高成骨细胞碱性磷酸酶的活性表達，并加速礦化结節的形成，從而完成成骨细胞的快速增殖舆分化和工程化组織的三维構建。所構建出的组織替代物具有很好的修復骨缺损的能力。In vitro fabrication of tissue-engineered bones with the BDBS （bio-derived bone scaffolds） in RWVB （rotating wall vessel bioreactor） and transplantation by using these constructs to repair the segmental bone defects of Zelanian rabbit were studied. In this paper, the osteoblasts, after being transfected with green fluorescent protein （GFP）, were seeded at 1 x 106 cells/mL onto the bio-derived bone scaffolds and then cultured in RWVB for one week. For a comparative study, cell seeded constructs were also cultured in static conditions. The morphology and structure of the fabricated bones were investigated by inverted microscope, SEM （scanning electron microscope）, and stains of HE （haematoxylin-eosin） and toluidine blue. Then, the animal experiment in repairing segmental bone defects of Zelanian rabbit by using the above tissue-engineered bone fabricated in RWVB was operated. The results showed that the cell number cultured in RWVB was five times as that in T-flask. These tissue-engineered bones cultured in RWVB grew well. The bone defects were almost repaired in the experimental group 8 weeks after the implantation, showing the best results among the 3 groups. It demonstrated that with the stress stimulation in RWVB, the ALP expression can be increased, a rapid proliferation and differentiation of osteoblasts are possible and the 3D fabrication of tissue-engineered bones could be realized. The tissue-engineered bone constructs fabricated in RWVB possess good ability in repairing segmental bone defect.

关 键 词：生物衍生骨 成骨細胞 構建 生物反應器 修復 

分 类 号：Q[生物学]