CD_(14)抑制肽的体外生物活性  

Biological activity of CD_(14) inhibitory peptide in vitro

作　　者：冯起甲[1] 徐智[1] 吴国明[1] 胡川闽[2] 徐顺贵[1] 钱桂生[1] 

机构地区：[1]第三军医大学附属新桥医院全军呼吸内科研究所全军呼吸病研究重点实验室,重庆400037 [2]第三军医大学医学检验系临床生物化学教研室,重庆400038

出　　处：《中华肺部疾病杂志（电子版）》2008年第1期107-110,共4页Chinese Journal of Lung Diseases(Electronic Edition)

摘　　要：目的研究CD14抑制肽(CD14-IP)与CD14的结合活性及对内毒素(LPS)和脂多糖结合蛋白(LBP)诱导的人单核巨噬细胞株U937表达肿瘤坏死因子-α(TNF-α)的影响。方法采用酶联免疫吸附(ELISA)法,进行CD14-IP与CD14的结合实验。U937细胞用佛波脂(PMA)诱导成熟后分为5组:正常对照组、LBP组(100 ng/ml LPS+100 ng/ml rhLBP)、高剂量多肽组、中剂量多肽组及低剂量多肽组,后3组分别给予10μg/ml、1.0μg/ml和0.1μg/ml的CD14-IP。用RT-PCR测定U937细胞TNF-αmRNA的表达,用ELISA测定培养上清液TNF-α的含量。结果CD14-IP有较强的与CD14结合的能力;CD14-IP组TNF-αmRNA的水平和TNF-α的浓度均较LBP组低,较正常组高。结论CD14-IP能与CD14结合,并能降低TNF-αmRNA和TNF-α的表达,可能对LPS所致急性肺损伤有潜在的治疗作用。Objective To investigate the binding efficacy of CD14 inhibitory peptide（CD14-IP） to CD14 and the effects of CD14-IP on TNF-α expression in U937 cells induced by LPS.Methods The binding test of CD14-IP to CD14 was carried out by ELISA.Then,U937 cells were stimulated by Phorbol myristate acetate（PMA） and divided into five groups： control group,LBP group（100 ng/ml LPS plus 100 ng/ml rhLBP）,high-dose inhibitory peptide group,middle-dose inhibitory peptide group,and low-dose inhibitory peptide group（10 μg/ml,1.0 μg/ml,and 0.1 μg/ml,respectively）.The mRNA of TNF-α in U937 cells was determined by RT-PCR and the concentration of TNF-α released from cells was detected by ELISA.Results CD14-IP has a high binding efficacy to CD14.The level of TNF-α mRNA and the concentration of TNF-α in inhibitory peptide group were lower than those in the LBP group,and were higher than those in the control group.Conclusion CD14-IP has a high binding efficacy to CD14,and can inhibit the expression levels of TNF-α mRNA and TNF-α.It has a potential protective effect on LPS induced inflammatory disorders such as acute lung injury.

关 键 词：CD14 多肽 脂多糖结合蛋白 TNF-Α 脂多糖 

分 类 号：R563.8[医药卫生—呼吸系统] R392.11[医药卫生—内科学]