胡椒属药材核糖体DNA的ITS序列分析及其分子鉴定  

Identification of Medicinal Plants from Piper L. Based on rDNA ITS Sequences

作　　者：蔡诚诚[1] 杨志业[1] 谢晖[1] 潘胜利[1] 

机构地区：[1]复旦大学药学院生药学教研室,上海201203

出　　处：《中国临床医学》2011年第2期136-138,共3页Chinese Journal of Clinical Medicine

基　　金：国家科技部重大专项"重大新药创制"(编号:2009ZX09103-075);上海市科委中药现代化专项(编号:08DZ1971203)

摘　　要：目的:研究核糖体DNA(rDNA)的ITS序列在胡椒属大叶蒟及其近缘药用植物及药材鉴定中的适用性。方法:聚合酶链反应(Polymerase Chain Reaction,PCR)扩增ITS序列测序,用DNAssist version2.2软件进行序列比对,并计算同源性。结果:胡椒属药材ITS序列与外类群ITS序列比对同源性均小于67.0%,属内序列间两两比对同源性均大于82.0%,同种植物则大于99.0%。结论:ITS序列作为胡椒属药材鉴定依据具有一定的适用性和可靠性。Objective： Study on the applicability of rDNA ITS sequence in identification of medicinal plants from Piper L.and its related species.Methods： The ITS regions of these species were amplified by PCR and sequenced,and their sequences were analyzed by DN Assist Version 2.2.Results：Homologous alignment indicated that the consanguinity of the ITS sequences between Genus Piper and outgroups was lower than 67.0%,and that within genus Piper was higher than 82.0%.For the same species,the consanguinity was higher than 99.0%.Conclusions： The ITS sequences may serve as reliable molecular markers for identification of Genus Piper and its related species.

关 键 词：胡椒属 ITS序列鉴定 

分 类 号：Q946[生物学—植物学]