急性发作期和缓解期哮喘患者外周血CD4^+CD25^+CD127^(lo/-)Treg的检测和意义  

The Detection and Significance of CD4^+CD25^+CD127^(lo/-)Tregs in Peripheral Blood CD4+T Cells of Healthy and Asthma Patients during Exacerbation and Remission

作　　者：上官文姬[1] 沈惠风[1] 王利民[1] 叶人诵[2] 

机构地区：[1]上海交通大学医学院附属仁济医院中医科中心实验室,上海200127 [2]上海市第八人民医院老年科,上海200233

出　　处：《中国临床医学》2011年第2期139-141,共3页Chinese Journal of Clinical Medicine

基　　金：上海市卫生局基金资助项目(编号:2008L033A)

摘　　要：目的:通过检测健康人、急性发作期和缓解期哮喘患者外周血CD4+CD25+CD127lo/-Treg细胞的表达水平,探讨该细胞表达水平的变化与哮喘患者病情严重程度的关系。方法:采集急性发作期、缓解期哮喘患者及健康人外周抗凝血,用流式细胞术检测其外周血中CD4+CD25+CD127lo/-Treg细胞占CD4+T细胞的比例。结果:哮喘急性发作组和缓解组患者外周血中CD4+CD25+CD127lo/-Treg细胞占CD4+T细胞的百分比低于健康对照组(P<0.05);哮喘急性发作期组CD4+CD25+CD127lo/-Treg细胞占CD4+T细胞的百分比低于哮喘缓解组(P<0.05)。结论:CD4+CD25+CD127lo/-Treg细胞数量减少可能参与了哮喘的发病过程。Objective：To investigate the relationship between the expression levels of CD4^＋CD25^＋CD127^lo/-Tregs in peripheral blood of asthma patients during exacerbation or remission. Methods： A total of 2 ml peripheral blood were collected in healthy or asthma patients during exacerbation and remission. The percentage of CD4^＋CD25^＋CD127^lo/-Tregs in CD4^ ＋ T cells by flow cy tometry were detected. Results： A significant decrease of the percentage of CD4^＋CD25^＋CD127^lo/-Tregs in CD4^＋ T cells was observed in exacerbation and remission groups compared with control group （P〈0.05）. This was also detected in exacerbation group compared with remission group （P〈0.05）. Conclusions： The CD4^＋CD25^＋CD127^lo/-Tregs play a key role in the pathogenesis of asthma and its decrease,which may lead to immune dysfunction, may be involved in the mechanisms of asthma.

关 键 词：支气管哮喘 CD4 CD25 CD127 调节性T细胞 

分 类 号：R566.25[医药卫生—呼吸系统]