腹腔注射硫酸镍对SD大鼠幼鼠半数致死量的研究  

Approach for LD50 of SD juvenile rats by intraperitoneal injection of nickel sulphate

作　　者：史才财[1] 张兴儒[1] 李青松[1] 周欢明[1] 项敏泓[1] 

机构地区：[1]上海中医药大学附属普陀医院眼科,中国上海市200040

出　　处：《国际眼科杂志》2011年第5期785-787,共3页International Eye Science

摘　　要：目的:观察硫酸镍腹腔注射对SD大鼠幼鼠的急性毒性作用,测定半数致死量(LD50)。方法:通过估摸实验、预初实验测出SD大鼠幼鼠硫酸镍的LD100为49.00mg/kg,LD0为25.00mg/kg,再用70只SD大鼠幼鼠,随机分成5组,腹腔注射2g/L硫酸镍,每组剂量分别为25.00mg/kg,29.58mg/kg,35.00mg/kg,41.39mg/kg,49.00mg/kg,按1.183倍递增。用SPSS16.0软件Bliss法测定腹腔注射硫酸镍LD50。结果:各组SD大鼠幼鼠在注射不同剂量硫酸镍后出现中毒反应表现,70只幼鼠中39只死亡,死亡率为55.7%,其中11只幼鼠在48h内死亡,其余28只在接下来的5d内相继死亡。SD大鼠幼鼠腹腔注射硫酸镍LD50为33.476mg/kg,95%可信区间为33.476±1.525mg/kg。结论:SD大鼠幼鼠腹腔注射硫酸镍LD50为33.476mg/kg,具有可信性。·AIM：To observe the acute toxicity of nickel sulphate in SD juvenile rat,and to determine the LD50.·METHODS：The LD100 in rat by ip injection was preliminarily measured to be 49.00mg/kg,and LD0 was 25.00mg/kg,then 70 rats were divided into 5 groups randomly,the doses of every group were 25.00mg/kg,29.58mg/kg,35.00mg/kg,41.39mg/kg,49.00mg/kg,the interval between groups was set at 1.183∶1,software of SPSS 16.0 was used to calculate its LD50.·RESULTS：The rats of every group showed poisoning reflection.39 rats died,the mortality rate was 55.7%,and 11 rats died in 48 hours,the remaining 28 died in the next 5 days.The LD50 of nickel sulphate in juvenile rats by ip injection was 33.476mg/kg,with the 95% confidence interval of 33.476±1.525mg/kg.·CONCLUSION：The LD50 of SD juvenile rats with ip injection of nickel sulphate is 33.476mg/kg,which is credible.·

关 键 词：硫酸镍 SD大鼠幼鼠 腹腔注射 半数致死量 

分 类 号：R965[医药卫生—药理学]