葛根素对糖尿病视网膜病变中Bcl-2表达的影响  

Effect of puerarin on expression of Bcl-2 in the retina of diabetic rats and the possible mechanism

作　　者：姚鹏[1] 杨惠婷[1] 滕岩[1] 刘海峰[1] 遇颖[1] 杨明明[1] 

机构地区：[1]牡丹江医学院附属红旗医院眼科,中国黑龙江省牡丹江市157011

出　　处：《国际眼科杂志》2011年第5期788-791,共4页International Eye Science

摘　　要：目的:通过分析凋亡细胞及凋亡通路中的关键因子Bcl-2表达的变化,阐明葛根素抗凋亡的作用和机制。方法:取成年健康大鼠60只,随机分为空白对照组、糖尿病(DM)组和DM葛根素干预组,ip链脲佐菌素建立DM大鼠视网膜病变模型,3mo时处死大鼠观察各组视网膜组织病理变化,并应用RT-PCR对Bcl-2进行定量分析。结果:DM大鼠HE染色可见神经节细胞减少,内外颗粒层平均光密度减低,有空泡形成,细胞排列紊乱稀疏。葛根素干预组细胞结构好转,未见空泡。RT-PCR可见Bcl-2在葛根素干预组表达明显高于正常组和DM组(P<0.05)。结论:葛根素可改善视网膜组织病理学变化,并增强Bcl-2的表达。·AIM：To investigate the effect of puerarin on apoptosis expression of Bcl-2 in the retina of diabetic rats and the possible mechanism of puerarin.·METHODS：Totally 60 male Wister rats were selected and divided at random into 3 groups：normal group,diabetic group and diabetic with puerarin injection group.The diabetic retinopathy rats model was established by injecting streptozotocin（STZ）.The pathogenic changes of diabetic retinopathy were observed and a quantitative analysis was given to Bcl-2 with RT-PCR at month 1,3 and 5,the data were analyzed with software SPSS 13.0.·RESULTS：Bcl-2 expressed in the retina of the normal rats.When at month 3,the expression of Bcl-2 was remarkably increased in diabetic group by puerarin injecting comparing with diabetic group（P0.05）,while at month 5,the expression of Bcl-2 was increased in the two groups.The pathologic damage in the retina of diabetic groups by puerarin injecting was slighter than diabetic groups.·CONCLUSION：Puerarin can alleviate the retinal pathological damage of diabetic rats.At month 3,Bcl-2 is expressed higher in the diabetic model by puerarin injecting than the diabetic group.Puerarin can increase the process of diabetic retinopathy by restraining the expression of Bcl-2.Consequently,puerarin will probably become the effective medicine to cure diabetic retinopathy.·

关 键 词：葛根素 糖尿病视网膜病变 BCL-2 细胞凋亡 凋亡机制 

分 类 号：R285.5[医药卫生—中药学]