ACS患者单核细胞MMP-9及其抑制物TIMP-1 mRNA的表达  

The expressions of MMP-9 and TIMP-1 mRNA in circulating monocytes of patients with ACS

作　　者：李如意[1] 于薇[1] 曹东平[1] 齐晓勇[1] 

机构地区：[1]河北省人民医院心脏中心,河北石家庄050051

出　　处：《中国老年学杂志》2011年第8期1305-1307,共3页Chinese Journal of Gerontology

基　　金：河北省卫生厅医学科学研究重点课题(No.08016)

摘　　要：目的探讨单核细胞基质金属蛋白酶-9(MMP-9)及组织型基质金属蛋白酶抑制物-1(TIMP-1)mRNA表达与急性冠脉综合征(ACS)之间的关系。方法分离8例ACS、8例稳定性心绞痛(SAP)及8例对照循环血单核细胞,采用异硫氰酸胍-酚-氯仿抽提法提取总RNA,扩增目的基因MMP-9及TIMP-1 mRNA。根据冠脉造影进行冠脉狭窄程度计分并测定血清ox-LDL水平。结果①ACS患者外周血单核细胞MMP-9 mRNA表达与对照组比较增加(P<0.05),而TIMP-1 mRNA表达在各组间无差异;②ACS组血清ox-LDL浓度高于SAP组及对照组(P<0.05),且SAP组高于对照组(P<0.05),③单核细胞MMP-9 mRNA表达与血清ox-LDL浓度正相关(r=0.458,P=0.024);而与冠脉狭窄计分(CSS)无相关性。结论 ACS患者外周血单核细胞MMP-9、TIMP-1 mRNA表达比例失衡,可能直接参与了不稳定斑块的形成及破裂。Objective To study the relationship between the expressions of MMP-9 and TIMP-1 mRNA in circulating monocytes and acute coronary syndrome（ACS）.Methods By the same method,circulating monocytes were separated from 8 patients with ACS,8 patients with stable angina pectoris（SAP） and 8 persons without coronary heart disease（CHD）.Then the total RNAs was isolated by acid guanidium thiocyanate-phenol-chloroform method from these cells and the expressions of MMP-9 and TIMP-1 mRNA were examined by RT-PCR.Furthermore the coronary stenosis was scored according coronary arteriography and the value of serum ox-LDL was detected.Results The expression of MMP-9 mRNA in monocytes of ACS patients was increased significantly compared to the patients with SAP and controls（P0.05）.But there was not significant difference in expression of TIMP-1 mRNA.The serum level of ox-LDL in ACS patients was increased significantly compared to the patients with SAP and controls（P0.05）.Furthermore the serum level of ox-LDL in ASP patients was also increased significantly compared to controls（P0.05）.The expression of MMP-9 mRNA was correlated with serum ox-LDL level（r=0.458,P=0.024）,but not with the extent of atherosclerotic lesions.Conclusions The imbalance expressions of MMP-9 mRNA and TIMP-1 mRNA could be involved in the development and disruption of unstable plaque.

关 键 词：急性冠脉综合征 单核细胞 基质金属蛋白酶-9 组织型基质金属蛋白酶抑制物-1 氧化低密度脂蛋白 

分 类 号：R541.4[医药卫生—心血管疾病]