重庆地区儿童中人冠状病毒流行情况分析  

Prevalence of Human Coronavirus in Children in Chongqing Area,China

作　　者：谢燕丕[1] 陈昕[1] 杜丽娜[1] 佘微微[1] 李荣培[1] 赵晓东[1] 

机构地区：[1]重庆医科大学附属儿童医院P2实验室,重庆400014

出　　处：《中国生物制品学杂志》2011年第4期464-468,共5页Chinese Journal of Biologicals

摘　　要：目的了解重庆地区住院患儿所患急性呼吸道感染与人冠状病毒(Human coronavirus,HCoV)HCoV-HKU1、HCoV-NL63、HCoV-OC43和HCoV-229E的相关性,并分析这4种HCoV的流行特征。方法采集2007年4月～2009年3月于重庆医科大学附属儿童医院因急性呼吸道感染住院的996份患儿的鼻咽部分泌物,经RT-PCR检测排除呼吸道合胞病毒(Respiratory syncytial virus,RSV)和人偏肺病毒(Human metapneumovirus,hMPV)阳性标本460份,采用RT-PCR法对536份RSV和hMPV阴性标本进行HCoV检测。结果 536份标本中共检出HCoV阳性10份,阳性率为1.86%,其中HCoV-HKU1 3份(0.56%),HCoV-NL63 4份(0.74%),HCoV-OC43 3份(0.56%),HCoV-229E 0份;10例患儿中男性9例,女性1例,年龄分布为0～5个月6例(60%),6～11个月2例(20%),12～23个月1例(10%),2～3岁1例(10%);3岁以上的未发现。HCoV感染呈全年散发,其中HCoV-HKU1感染集中在冬春季,HCoV-NL63和HCoV-OC43感染集中在夏秋季。HCoV感染的临床症状表现为发热、咳嗽、痰、喘息、呕吐和腹泻;临床诊断为支气管肺炎4例,间质性肺炎3例,迁延性肺炎1例,毛细支气管炎1例、急性喉气管支气管炎1例,其中有6例合并症状性腹泻。结论重庆地区因急性呼吸道感染住院的患儿中HCoV感染率较低,HCoV可引起下呼吸道感染和消化道感染。Objective To investigate the prevalence and epidemic features of four kinds of human coronavirus（HCoV）,i.e.HCoV-HKU1,HCoV-NL63,HCoV-OC43 and HCoV-229E in hospitalized children with acute respiratory tract infection（ARTI） in Chongqing Area,China.Methods Nasopharyngeal aspirates（NPAs） were collected from 996 hospitalized children with ARTI in Children＇s Hospital of Chongqing Medical University from April 2007 to March 2009,of which 460 specimens positive for respiratory syncytial virus（RSV） and human metapneumovirus（hMPV） as proved by RT-PCR were excluded,while the other 536 specimens negative for RSV and hMPV were determined for HCoV.Results Ten（1.86%） of the 536 specimens were proved as HCoV-positive,of which 3（0.56%） were positive for HCoV-HKU1,4（0.74%） for HCoV-NL63,3（0.56%） for HCoV-OC43 and 0 for HCoV-229E.Of the 10 children,9 were male and 1 was female,6（60%） were at ages of 0 ~ 5 months,2（20%） at 6 ~ 11 months,1（10%） at 12 ~ 23 months,1 at 2 ~ 3 years,while none at age of more than 3 years.HCoV infection was sporadic in the whole year,of which HCoV-HKU1 infection mainly occurred in winter and spring,while HCoV-NL63 and HCoV-OC43 in summer and fall.The clinical symptoms of HCoV infection included fever,cough,sputum,pant,vomiting and diarrhea.Four of the 10 cases were diagnosed as bronchopneumonia,3 as interstitial pneumonia,1 as persistent pneumonia,1 as capillary bronchitis,and 1 as acute laryngotracheal bronchitis,of which 6 were complicated with symptomatic diarrhea.Conclusion HCoV was low prevalent in the hospitalized children with ARTI in Chongqing Area,which might cause infections in lower respiratory tract and alimentary canal.

关 键 词：冠状病毒属 HCoV-HKU1 HCOV-NL63 流行病学 

分 类 号：R373.9[医药卫生—病原生物学]