免疫应答基因与小鼠膜性肾小球肾炎发病的关系  

The study of immune response gene and the mechanism of the membranous glomerulonephritis in the mouse

作　　者：张虎祥[1] 张海燕[2] 万丽[1] 马跃荣 

机构地区：[1]温州医学院附属第一医院病理科,浙江省温州325000 [2]温州医学院附属第一医院急诊科,浙江省温州325000 [3]不详

出　　处：《中国基层医药》2011年第5期602-604,I0001,共4页Chinese Journal of Primary Medicine and Pharmacy

基　　金：浙江省温州市科技局基金项目（Y20090041）

摘　　要：目的 探讨免疫应答基因（I-Aβ1）与小鼠膜性肾小球肾炎发病之间的相关性.方法 复制小鼠膜性肾小球肾炎模型,提取实验组和对照组总RNA,通过RT-PCR体外扩增I-Aβ1基因,对扩增产物进行测序及分析.结果 实验组小鼠I-Aβ1基因突变率为2.578‰,对照组I-Aβ1基因突变率为0.286‰,实验组I-Aβ1基因突变率明显高于对照组（P＜0.01）.结论 I-Aβ1可能与小鼠膜性肾小球肾炎发病相关.Objective To study the immune response gene and the mechanism of membranous glomerulonephritis in the mouse. Methods Reproduced and identitied the animal model of MGN in mice ,extracted the total RNA of pathology group and the control group,amplified and verified I-Aβ1 gene through RT-PCR. Then sequenced and analyzed the I-AβI gene from the PCR production. Results The mutation rate of the I-Aβ1 gene was 2. 578‰ in the pathology group, and 0. 286‰ in the control group. It was obviously higher in the pathology group than in the control group（P ＜0.01）. Conclusion I-Aβ1 gene rose in mice,may be related to membranous glomerulonephritis.

关 键 词：基因 MHCⅡ类 逆转录聚合酶链反应 肾小球肾炎 膜性 

分 类 号：R692[医药卫生—泌尿科学]