三个血清学标志物联合检测用于肝癌诊断的临床意义  

Clinical Significance of 3 Serum Markers in Combined Diagnosis of Hepatic Carcinoma

作　　者：陈浩[1] 张明跃[1] 张桂珍[1] 曹明[1] 丁磊[1] 

机构地区：[1]河北省保定市传染病医院临床基因扩增检验实验室,071000

出　　处：《职业与健康》2011年第9期1001-1002,共2页Occupation and Health

摘　　要：目的通过对原发性肝癌以及良性肝病患者样本进行甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)以及高尔基体蛋白73(GP73)3种血清学标志物的检测,研究3种血清学肿瘤标志物指标联合检测用于诊断肝癌的临床意义。方法采用酶联免疫法检测GP73,用化学发光法检测AFP,AFP检测为阳性的再采用亲和吸附离心法检测AFP-L3。结果 AFP、AFP-L3、GP73联合用于检测可以使肝癌诊断的敏感性提高到96.72%(118/122),将肝癌诊断的特异性提高到96.05%(73/76)。结论将AFP、AFP-L3以及GP73用于联合检测,可以有效地提高目前临床检测肝癌的敏感性以及特异性。[Objective]To explore the clinical significance of alpha fetoprotein（AFP）,AFP variants（AFP-L3） and Golgi protein-73（GP73） in diagnosing primary hepatic carcinoma（PHC）,through the detection of above 3 serum markers in patients with PHC and benign liver disease.[Methods],GP73 was detected by ELISA,AFP was detected by Chemiluminescent Immunoassay,AFP positive samples was detected for AFP L3 by centrifugal adsorption affinity.[Results]the combined detection of AFP,AFP-L3,GP73 could increase the diagnostic sensitivity of primary hepatic carcinoma to 96.72%（118/122） and the specificity to 96.05%（73/76）.[Conclusion]The combination of AFP,AFP-L3,GP73 can effectively improve the current clinical sensitivity and specificity of diagnosis of primary hepatic carcinoma

关 键 词：甲胎蛋白 甲胎蛋白异质体 高尔基体蛋白73 肝癌 

分 类 号：R446[医药卫生—诊断学]