电磁辐射急性暴露对Th1/Th2细胞因子平衡的影响  

Electromagnetic acute irradiation leads to Th1/Th2 cytokine imbalance in mice

作　　者：王源[1] 许商成[1] 张广斌[1] 储芳[1] 余争平[1] 

机构地区：[1]第三军医大学军事预防医学院劳动卫生学教研室,电磁辐射医学防护教育部重点实验室,重庆400038

出　　处：《第三军医大学学报》2011年第9期883-886,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30972432)~~

摘　　要：目的观察电磁辐射对Th1/Th2细胞因子平衡的影响。方法以平均功率密度为90 mW/cm2的微波辐照昆明小鼠（4~6周龄,体质量20~25 g,40只,均为雌性）20 min,采用RT-PCR、ELISA检测微波辐照后不同时相点干扰素（interferon-gamma,INF-γ）、白细胞介素-4（interleukin-4,IL-4）表达量的变化,分析微波辐照对Th1/Th2平衡的偏移。结果 90 mW/cm2的微波急性辐照会导致INF-γ表达量的增高,辐照后第5天达到峰值,增幅为135.5%（P〈0.01）;90 mW/cm2的微波急性辐照使IL-4的表达有降低的趋势,但与对照组相比没有显著差异（P〉0.05）。结论平均功率密度为90 mW/cm2的微波急性辐照会导致昆明种小鼠Th1/Th2平衡向Th1方向偏移。Objective To identify the effect of electromagnetic irradiation on Th1/Th2 cytokine balance.Methods A total of 40 Kunming mice were randomly divided into sham control（n=5）,and irradiation groups（1,2,3,5,7,9 and 11 d after 90 mW/cm2 electromagnetic irradiation for 20 min,n=5 for each time point）.The mice were sacrificed at the above correspondingly time points respectively after exposure,and their blood samples and spleen tissues were collected.The expression levels of interferon-gamma（INF-γ） and interleukin-4（IL-4） were detected with RT-PCR for spleen tissues and ELISA for blood samples.The excursion of Th1/Th2 cytokine balance induced by electromagnetic irradiation was analyzed.Results INF-γ expression was obviously raised by 90 mW/cm2 electromagnetic irradiation.Its serum level reached a a peak in 5 d after exposure,with an increase of 135.5% compared with sham control group（P0.01）.IL-4 expression had a drawdown tendency by 90 mW/cm2 electromagnetic irradiation,but it had no significant difference when compared with sham control（P0.05）.Conclusion Electromagnetic irradiation at 90 mW/cm2 leads to the Th1/Th2 cytokine balance deflect to Th1.

关 键 词：电磁辐射 INF- γIL-4 TH1/TH2平衡 

分 类 号：R818.023[医药卫生—放射医学] R392.11[医药卫生—临床医学]