PI3K/Akt信号转导通路与卵巢癌顺铂耐药相关性的研究  

Study on the Relationship between PI3K/Akt Signal Transduction Pathway and Cisplatin Resistance in Ovarian Carcinoma

作　　者：张栋[1] 孔德璇[1] 和珍珍[1] 瞿全新[1] 

机构地区：[1]天津医科大学一中心临床学院妇产科,300192

出　　处：《天津医药》2011年第4期361-363,共3页Tianjin Medical Journal

摘　　要：目的:研究卵巢癌顺铂(DDP)耐药与磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号转导通路的关系。方法:根据LY294002作用浓度将SKOV3和SKOV3/DDP细胞分别分为5μmol/L+DDP、10μmol/L+DDP、20μmol/L+DDP、40μmol/L+DDP、DDP组及对照组。MTT法检测40μmol/L LY294002对SKOV3和SKOV3/DDP细胞DDP敏感性的影响;Western blot法检测SKOV3和SKOV3/DDP细胞各实验组PI3K、Akt表达水平。结果:SKOV3和SKOV3/DDP细胞经LY29400240μmol/L作用后,对DDP敏感性分别增加了67.36%、76.56%。LY294002可降低SKOV3和SKOV3/DDP细胞中PI3K和Akt表达。SKOV3细胞对照组PI3K和Akt蛋白相对浓度值均低于SKOV3/DDP细胞对照组(t分别为26.005和23.477,均P<0.05)。结论:PI3K/Akt信号通路异常与卵巢癌DDP耐药有关,抑制PI3K/Akt信号通路能增强卵巢癌DDP敏感性。Objective： To investigate the relationship between cisplatin （DDP） resistance of ovarian carcinoma and PI3K/Akt signal transduction pathway. Methods： SKOV3 and SKOV3/DDP cells were divided into 6 groups depending on the concentration of LY294002, which were 5 μmol/L,10 μmol/L,20 μmol/L,40 μmol/L,DDP and control group. MTT assay was used to analyze the effect of 40 μmol/L LY294002 on the sensitivity of SKOV3 and SKOV3/DDP. Western blot was used to detect the expressions of PI3K, Akt and β-actin in SKOV3 and SKOV3/DDP cells of each group. Results： After interference by 40 μmol/L of LY294002, the sensitivity to DDP of SKOV3 and SKOV3/DDP cells increased 67.36% and 76.56%. The expressions of PI3K and Akt in SKOV3 and SKOV3/DDP cells were reduced by LY294002. The correspondence densities of PI3K and Akt were lower in SKOV3 cells than those in SKOV3/DDP cells （t = 26.005 and t = 23.477,P 0.05）.Conclusion： The abnormal PI3K/Akt signaling pathway is related to cisplatin resistance in ovarian carcinoma. The chemosensitivity of ovarian cancer can be enhanced by inhibiting PI3K/Akt signaling pathway.

关 键 词：卵巢肿瘤 癌 顺铂 抗药性 肿瘤 细胞系 肿瘤 1-磷脂酰肌醇3-激酶 蛋白激酶类 

分 类 号：R335.6[医药卫生—人体生理学]