矽肺患者氧化应激指标及外周血单核细胞NF-κB水平的变化  

Effects of oxidative stress and NF-κB levels in peripheral blood mononuclear cells on development of silicosis

作　　者：张雪涛[1] 倪为民[1] 缪荣明 刘锡诚 鲁翼雯[1] 杨水莲[1] 朱玮 卞红[1] 匡兴亚[1] 姚峰[1] 

机构地区：[1]上海市杨浦区中心医院职业病科,200090 [2]江苏省无锡市职业病防治院 [3]黑龙江省第二医院 [4]无锡市锡山区卫生监督所

出　　处：《中华劳动卫生职业病杂志》2011年第4期251-254,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：上海卫生局科研课题（2007029）

摘　　要：目的 观察矽肺患者氧化应激指标及外周血单核细胞NF-κB水平的变化,探讨矽肺发生发展的机制.方法 选择某铸造厂接触矽尘作业工龄在1年以上的工人200例为接尘组,该厂2008年住院及门诊随访的矽肺患者130例为矽肺组,32例0＋病例为观察对象组,同时选择某酒店服务人员100例为对照组.分别测定超氧化物歧化酶（SOD）、血清中谷胱甘肽过氧化物酶（GSH-Px）、一氧化氮合酶（NOS）活力,一氧化氮（NO）、丙二醛（MDA）含量及总抗氧化能力（T-AOC）,外周血单核细胞核蛋白中NF-κB水平.结果 与对照组比较,接尘组和矽肺组NO含量明显升高,SOD活力明显降低,差异均有统计学意义（P＜0.01）.与对照组及接尘组比较,矽肺组T-AOC水平、NOS活力、MDA含量均明显升高,差异均有统计学意义（P＜0.01）.与对照组[（223.360±46.838）U/ml]比较,接尘组及矽肺组GSH-Px活力[（231.164±36.484）、（270.469±39.228）U/ml]明显升高,且矽肺组GSH-Px活力明显高于接尘组,差异均有统计学意义（P＜0.05,P＜0.01）.与观察对象组[（256.906±21.418）U/ml]和Ⅰ期矽肺组[（259.594±34.790）U/ml]比较,Ⅲ期矽肺组GSH-Px活力[（290.750±39.129）U/ml]明显升高,差异均有统计学意义（P＜0.05）.与对照组[（59.71±9.27）ng/L]比较,接尘组及矽肺组NF-κB水平[（72.06±9.12）、（85.25±11.64）ng/L]明显升高,且矽肺组NF-κB水平明显高于接尘组,差异均有统计学意义（P＜0.01）.血清中GSH-Px活力与矽肺分期呈正相关（r=0.507,P＜0.01）.外周血单核细胞核蛋白NF-κB水平与矽肺分期、年龄、GSH-Px活力、NO含量呈正相关,差异均有统计学意义（r值分别为0.376、0.243、0.233、0.221,P＜0.01）.结论 机体氧化和抗氧化系统的失衡与矽肺的发生发展有关,并与NF-κB的活化一致.Objective To investigate the change of indicators of oxidative stress in serum and NF-κB in peripheral blood mononuclear cells of patients with silicosis, and explore the mechanism of the development of silicosis. Methods The subjects were divided into （1） 200 workers exposed to SiO2 for at least 1 years in a foundry served as the dust-exposure group; （2） 130 cases with silicosis （Ⅰ phase silicosis 64 cases, Ⅱ phase 46 cases Ⅲ phase 20 cases） served as the silicosis goup; （3） 32 cases with 0＋ phase silicosis in the foundry served as the observed group,（4）100 subjects from a hotel served as the control group. The serum including superoxide dismutase （SOD）, nitric oxide （NO）, serum glutathione peroxidase （GSH-Px）, total antioxidant capacity （T-AOC）, nitric oxide synthase （NOS）, lipid malondialdehyde （MDA） and NF-κB protein levels in peripheral blood mononuclear cells were determined, respectively. Results Compared with the control group,NO levels in dust-exposed group and silicosis group significantly increased, and SOD decreased significantly （P＜0.05 or P＜0.01）. Compared with the control group and dust-exposed group, T-AOC, NOS, MDA levels in silicosis group significantly increased （P＜0.05 or P＜0.01）. GSH-Px in dust-exposed group and silicosis group were （231.164±36.484） and （270.469±39.228）U/md, respectively which were significantly than that [（223.360±46.838） U/ml] in control group （P＜0.05 or P＜0.01）, and there was significant difference of GSHPx between the silicosis group and the dust-exposed group significantly （P＜0.01）. GSH-Px level [（290.750±39.129） U/ml] in Ⅲ phase silicosis group were significantly higher than those [（256.906±21.41） and （259.594±34.79） U/ml] in observation group and Ⅰ phase silicosis group （P＜0.05）. NF-κB levels [（72.06±9.12） and （85.25±11.64） ng/L] in dust-exposed group and silicosis group were significantly higher than that [（59.71±9.27） ng/L] in co

关 键 词：矽肺 超氧化物歧化酶 一氧化氮合酶 NF-ΚB 

分 类 号：R135.2[医药卫生—劳动卫生]