乌司他丁对百草枯染毒大鼠肺组织热休克蛋白70和NF-κB的影响  

Effect of ulilnastatin on the expression of heat shock protein 70 and NF-κB in lung tissue in rats with paraquat poisoned

作　　者：周从阳[1] 张志坚[2] 罗雅娟[1] 肖声平[1] 林时荣[1] 

机构地区：[1]南昌大学第一附属医院急诊科,330006 [2]重庆市巴南区人民医院ICU

出　　处：《中华劳动卫生职业病杂志》2011年第4期280-283,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的 观察急性百草枯（paraquat,PQ）染毒大鼠肺组织中热休克蛋白70（hsp70）及NF-κB mRNA表达及乌司他丁（ulilnastatin,UTI）的作用.方法 将72只SD大鼠随机分为对照组、PQ组和UTI组,每组24只.染毒组：以80 mg/lg PQ一次性灌胃;治疗组：PQ灌胃后以UTI 10000 U/kg腹腔注射,每日1次.对照组一次性灌胃生理盐水.于12、24、48、72 h分别测定肺组织中髓过氧化物酶（MPO）活力,反转录-聚合酶链反应（RT-PCR）法测定肺组织中hsp70和NF-κB p65 mRNA的表达,观察大鼠肺组织病理变化.结果 PQ染毒后48 h内炎性细胞浸润明显,伴充血水肿;UTI组炎性细胞浸润较PQ染毒组少,充血水肿轻.染毒组大鼠肺组织中MPO活力在12、24、48、72 h分别为（31.72±6.42）、（56.23±8.63）、（87.21±10.02）、（107.21±13.52）U/g,UTI组12、24、48、72 h MPO活力分别为（15.65±3.21）、（35.98±5.74）、（59.33±9.65）、（71.25±10.58）U/g,较对照组[（11.38±1.25）U/g]明显升高,差异有统计学意义（P＜0.01）;UTI组MPO活力较染毒组明显降低,差异有统计学意义（P＜0.01）.UTI组12、24、48、72 h NF-κB p65 mRNA表达分别为0.3288±0.0147、0.5337±0.0328、0.7357±0.0424、0.7547±0.0905,与PQ组（分别为0.41 85±0.0294、0.8532±0.0841、0.9554±0.0975、1.0094±0.0703）比较,明显降低,差异有统计学意义（P＜0.01）.UTI组12、24、48、72 h hsp70 mRNA表达分别为0.5193±0.0254、0.8289±0.0606、0.7566±0.0277、0.4873±0.0105,与PQ组（分别为0.3897±0.0125、0.5904±0.0186、0.4007±0.0237、0.2293±0.0137）比较明显增加,差异有统计学意义（P＜0.01）.结论 大鼠急性PQ中毒后肺组织中hsp70、NF-κB p65 mRNA表达明显增高;UTI可以通过增加PQ中毒大鼠肺组织中HSP70表达,降低NF-κB表达,对肺脏起保护作用.Objective To observe the expression levels of heat shock protein70 （hsp70） and NF-κB p65 mRNA in lung tissue of acute paraquat （PQ） poisoning rats, and intervention effects of ulinastatin （UTI）.Methods Seventy-two Sprague-Dawley （SD） rats were randomly divided into three groups： PQ poisoning group, UTI group and control group. The rats were exposed intragastrically to PQ at the dose of 80 mg/kg to establish a model of the rat acute lung injury. The UTI group was intervened by peritoneal injection with 10000 U/kg UTI in 30 minutes. On the 12, 24, 48, 72 h after exposure, myeloperoxidase （MPO） activity in lung tissue were detected. The expression of the NF-κBp 65 mRNA and hsp70 mRNA in lung tissue was detected by the reverse transcription-PCR （RT-PCR）.The lung pathological changes of rats were observed. Results The degree of lung injury in PQ group and UTI group was higher than that in control group. But in UTI group the degree of lung injury was lower than PQ group. MPO activity in the lung tissues in PQ group was （31.72±6.42）,（56.23±8.63）, （87.21 ±10.02） and （107.21 ±13.52） μ/g in 12, 24, 48 and 72 h, respectively which was significantly higher than that [（11.38±1.25） μ/g] in control group （P＜0.01）. MPO activity in the lung tissues in UTI group was （15.65±3.21）, （35.98±5.74）, （59.33±9.65） and （71.25±10.58） μ/g in 12, 24, 48 and 72 h,respectively which was significantly lower than those in PQ group （P＜0.01）. The expression levels of NF-κB p65 mRNA of lung tissues in UTI group in 12, 24, 48 and 72 h were 0.3288±0.0147, 0.5337±0.0328, 0.7357±0.0424 and 0.7547±0.0905, respectively, which were significantly lower that those （0.4185±0.0294, 0.8532±0.0841,0.9554±0.0975 and 1 .0094±0.0703） in PQ group （P＜0.01）. hsp70 mRNA expression levels in 12, 24,48 and 72 h of the UTI group were 0.5193±0.0254, 0.8289±0.0606, 0.7566±0.0277 and 0.4873±0.0105,respectively, which were significantly higher than those （0.3897±0

关 键 词：百草枯 乌司他丁 热休克蛋白质类70 NF-кb 

分 类 号：R5[医药卫生—内科学]