嗅鞘细胞联合甲基强的松龙对大鼠脊髓损伤区神经中丝表达的影响  

Expression of neurofilament in injured spinal cord zone in response to olfactory ensheating celltransplantation combined with methyprednisolone

作　　者：王斌[1] 贺西京[1] 历强[1] 

机构地区：[1]广州医学院第二附属医院创伤外科,510260

出　　处：《中华创伤杂志》2011年第4期370-374,共5页Chinese Journal of Trauma

基　　金：基金项目：广州医学院博士启动基金资助项目（3030050020364）;教育部博士学科点专项科研基金资助项目（20050698043）

摘　　要：目的 观察嗅鞘细胞（olfactory ensheating cells,OECs）移植联合甲基强的松龙（methyprednisolone,MP）对大鼠脊髓损伤区神经中丝（neurofilament,NF）mRNA及蛋白表达的影响,探讨OECs移植联合应用MP促进损伤脊髓修复的机制.方法 以NYU脊髓打击法建立大鼠急性T10脊髓损伤模型.按随机数字表法分为A组空白组10只;B组损伤组、C组DF12组、D组OECs组、E组MP组、F组OECs＋MP组,各30只.于治疗后不同时相点,取各组大鼠损伤区脊髓应用免疫组织化学染色、反转录PCR和Western blot的方法检测NF mRNA及蛋白表达变化.结果 OECs组、MP组、OECs＋MP组与损伤组、DF12组比较,术后7,14,28 d损伤区脊髓组织NFmRNA及蛋白表达显著升高（P＜0.05）.OECs＋MP组与OECs组、MP组比较,术后7,14,28d损伤区脊髓组织NF mRNA及蛋白表达显著升高（P＜0.05）.结论 OECs移植或单纯应用MP可促进大鼠脊髓损伤区促轴突再生因子NFmRNA及蛋白表达,二者联合应用,可显著促进脊髓损伤区NF mRNA及蛋白表达,具有协同作用效果.此结果提示OECs＋MP可调控NFmRNA及蛋白表达,且此调控作用可能是OECs＋MP促进脊髓损伤修复的可能机制之一.Objective To study the mRNA and protein expressions of the neurofilament in the region of acute spinal cord injury （SCI） of rats after olfactory ensheating cells （OECs） transplantation combined with methyprednisolone administration and investigate the molecular mechanisms of OECs transplantation combined with methyprednisolone administration in promoting the recovery of the spinal cord.Methods Acute spinal cord injury was established in SD rats （ T10 ） by using NYU instrument. The rats were randomly divided into control group, SCI group, DF12 group, OECs transplantation group,methyprednisolone administration group and OECs ＋ methyprcdnisolone group. The mRNA and protein expressions of the neurofilament in the SCI regions of rats in different groups at different time points were detected by using immunohistochemistry, RT-PCR and Western blotting. Results A significant increase of mRNA and protein expressions of the neurofilament could be found in the other five groups compared with the control group at days 7, 14 and 28 after SCI. The mRNA and protein expressions of the neurofilament in the injury region of the OECs group, the methyprednisolone group and the OECs ＋ MP group were more significantly increased than that of the SCI group and the DF12 group. The expression of the neurofilament in the injury region of the OECs ＋ MP group was more significantly increased than that of the OECs group and the MP group （ P 〈 0. 05）. Conclusions OECs transplantation or methyprednisolone administration can induce the mRNA and protein expressions of the neurofilament. Meanwhile, OECs transplantation combined with methyprednisolone administration can significantly increase the mRNA and protein expressions of the neurofilament, as may be one of mechanisms promoting spinal cord repair.

关 键 词：脊髓损伤 嗅鞘细胞 神经中丝蛋白 

分 类 号：R686[医药卫生—骨科学]