人肝脏星状细胞糖基化终产物受体表达的研究  

Expression of receptor for advanced glycosylation end products in cultured human hepatic stellate cells

作　　者：刘乃丰[1] BachemMG GruenertA 

机构地区：[1]南京铁道医学院附属医院心内科,南京210009 [2]德国乌尔姆大学医学临床化学研究所

出　　处：《铁道医学》1999年第5期288-290,共3页Railway Medical Journal

摘　　要：目的　研究人肝脏星状细胞（ ＨＳＣ） 上糖基化终产物受体（ＲＡＧＥ） 的表达。方法　用生物素将体外制备的糖基化终产物（ ＡＧＥｓ － ＢＳＡ） 标记成配基，以细胞化学法显示细胞表面受体高亲和性结合。用反转录聚合酶链反应（ ＲＴ－ＰＣＲ） 检测ＡＧＥｓ 受体基因的表达。结果　细胞化学法显示ＡＧＥｓ － ＢＳＡ 与ＨＳＣ 呈高亲和性结合，部分被内化。用按人ＲＡＧＥ 基因序列设计的引物，反转录扩增出２８３ ｂｐ ＤＮＡ 片段。结论　人肝脏星状细胞表达的ＡＧＥｓObjective To investigate expression of receptor for advanced glycosylation end products (AGEs) in cultured human hepatic stellate cells (HSC). Methods AGEs-BSA prepared in vitro was labeled with biotin to demonstrate binding of AGEs to receptor for AGEs (RAGE). High affinity binding of cell surface was shown with cell chemistry method. RT-PCR was performed with oligonuclotide primers according to human RAGE gene sequence to detect mRNA expression of RAGE. Results High affinity binding of biotin labeled AGEs-BSA to surface of cultured HSC was identified. 283 bp DNA was amplified which was consistent with predicted fragment according human RAGE sequence. Conclusion The results suggested that expression of RAGE in HSC might be of significance in pathogenesis of accelerated hepatic fibrosis in diabetic patients.

关 键 词：糖尿病 肝纤维化 肝脏星状细胞 RAGE 

分 类 号：R587.2[医药卫生—内分泌] R575.202[医药卫生—内科学]