兔颈动脉损伤后再狭窄与p53蛋白关系的研究  

The expression of p53 in preventing restenosis after rabbit carotid artery balloon injury

作　　者：蒋伟[1] 王书梅[2] 顾新元[1] 唐良秋[1] 

机构地区：[1]韶关市粤北人民医院心内科,广东省韶关512025 [2]韶关市粤北人民医院口腔科,广东省韶关512025

出　　处：《中国医师杂志》2011年第3期300-301,327,共3页Journal of Chinese Physician

基　　金：基金项目：广东省韶关市科技局计划项目（卫2009-07）

摘　　要：目的 探讨p53蛋白在血管损伤后再狭窄形成过程中的作用.方法 建立兔颈动脉球囊损伤模型,30只家兔随机分为两组：假手术组（n=6）、血管损伤后1、7、14及28 d组（n=24）.采用HE染色方法 观察血管内膜增生情况,免疫组化检测血管壁p53蛋白的表达.结果 假手术组p53的DD值为（20.02±4.86）×10-3,手术组中7、14、28 d的内膜及中膜的p53的DD值分别为（10.84±12、8.12±1.26、7.08±0.28）×10-3与（1.67±0.57、1.09±0.84、1.05±0.64）×10-3.与假手术组相比,球囊损伤后颈动脉内膜面积逐渐增加,血管壁p53表达减少（P＜0.01）,但在新生内膜层的p53表达水平高于中膜层（P＜0.01）.结论 p53参与了血管损伤后的修复过程,可能与PTCA术后血管再狭窄有关.Objective To observe the effect of p53 protein on smooth muscle cell（VSMC）in rabbit artery balloon injury.Methods Restenosis model of carotid artery after balloon injury was established in rabbits.30 rabbits were divided into 2 groups,the sham group（n = 6）and the vascular injury group（n = 24）.With H.E.staining and automatic image analysis system,we investigated artery morphology alteration and measured the area of arterial intima and media.The expressions of p53 protein were detected by immunohistochemical analysis.Results With H.E.staining and automatic image analysis and immunohistochemistry,the results showed that the expression of p53 was significantly reduced and the intima area was increased in model group compared with the sham group（P 〈0.01）.But the expression of p53 in media was remarkably reduced compared with intima（P 〈 0.01）.Conclusions The possible mechanism of preventing arterial restenosis in the balloon injury might be related with p53,which may be through inhibiting neointimal proliferation in arterial restenosis .

关 键 词：肿瘤抑制蛋白质p53/代谢 颈动脉损伤/并发症/代谢 缩窄 病理性/并发症/代 谢 血管成形术 经腔 经皮冠状动膨副作用 

分 类 号：R541.4[医药卫生—心血管疾病]