肠缺血再灌注损伤大鼠小肠运动功能的变化与细胞间黏附分子1表达的关系  

Intestinal motility alterations and expression of intercellular adhesion molecule-1 within the rat intestinal muscularis after ischemia reperfusion

作　　者：刘健[1] 赵战朝[1] 薛承锐[1] 

机构地区：[1]天津医科大学总医院外科,300052

出　　处：《中华普通外科杂志》2011年第4期328-331,共4页Chinese Journal of General Surgery

摘　　要：目的 探讨肠缺血再灌注（intestinal ischemia reperfusion,IIR）损伤大鼠小肠运动功能的变化及其与细胞间黏附分子1（intercellular adhesion molecule-1,ICAM-1）表达的关系.方法 将Wistar大鼠30只随机分为ⅡR组、ICAM-1单抗组和假手术组,ⅡR组采用夹闭-放开肠系膜上动脉的方法建立IIR损伤模型,ICAM-1单抗组于阻断肠系膜上动脉血供同时静脉给予ICAM-1单克隆抗体1 mg/kg.用荧光分光光度法测定各组大鼠小肠传输功能,多导生理仪测定环行肌条收缩能力的变化,RT-PCR法检测小肠肌层ICAM-1 mRNA的表达量,免疫组化法检测ICAM-1蛋白表达,图像分析系统计数小肠肌层浸润白细胞数量,比色法测定小肠肌层组织髓过氧化物酶（myeloperoxidase,MPO）的活性.结果 ICAM-1单抗组较ⅡR组大鼠小肠传输功能明显改善,几何中心分别为8.4±0.7和3.4±0.5;两组环形平滑肌条收缩力为（1.81±0.28）g/mm2·s-1和（0.52±0.09）g/mm2·s-1;浸润白细胞数量为（5.6±2.2）c/f和（18.2±3.1）c/f;MPO活性为（2.03±0.56）U/g和（6.41±1.25）U/g,两组各项指标之间比较差异均有统计学意义（P＜O.05）.两组小肠肌层ICAM-1 mRNA的表达量为1.69±0.57和1.76±0.32,差异无统计学意义（P＞0.05）.IIR组和ICAM-1单抗组小肠肌层均有大量棕色颗粒样染色.结论IIR损伤上调小肠肌层ICAM-1 mRNA的表达,介导中性粒细胞的黏附和浸润,导致小肠运动功能的障碍.Objective To evaluate the relationship between the intestinal motility alterations and intercellular adhesion molecule-1 （ ICAM-1 ） expression within the rat intestinal muscularis after ischemia reperfusion. Methods Thirty Wistar rats were divided randomly into IIR, monoclanal anti-ICAM-1 and sham group （n = 10）, HR models were established by clamping-releasing the superior mesenteric artery. Gut transit was measured in vivo and intestinal circular muscle contractions were measured in an organ bath. The expression of ICAM-1 mRNA was detected by RT-PCR and immunohistochemisty. Leukocyte infiltration and myeloperoxidase （MPO） activity were quantified in sham and ischemia/reperfusion rats with and without monoclonal anti-ICAM-1 antibody treatment. Results The gut transit of monoclonal anti-ICAM-1 group was improved obviously as compared with IIR group. Circular smooth muscle contractility stimulated by ICAM-1 mRNA expression was 1.69 ± 0. 57 and 1.76 ± 0. 32 within the muscularis; Leukocyte infiltration into the muscularis was （5.6 ±2. 2） c/f and （ 18. 2 ±3. 1 ） c/f. MPO activity was （2. 03 ±0. 56） U/g and （6. 41 ± 1.25 ） U/g respectively. The differences were all statistically significant between IIR and treatment groups （ P 〈 0. 05 ）. The expression of ICAM-1 protein in IIR and anti-ICAM-1 groups was not significantly different （P 〉 0. 05）. Conclusions The up-regulated expression of ICAM-1 after ⅡR injury calls forth local infiltration of PMN within the intestinal muscularis, leading to intestinal motility dysfunction.

关 键 词：缺血 再灌注损伤 疾病模型 动物 运动障碍 胞间黏附分子1 

分 类 号：R285.5[医药卫生—中药学]