TRAIL及其受体在自身免疫性炎性肌病大鼠肺组织中的表达  

Expression of TRAIL and its receptors in interstitial lung tissue of autoimmune myositis in rats

作　　者：赵华[1] 王国春[2] 

机构地区：[1]北京协和医学院研究生院,北京100730 [2]中日友好医院风湿免疫科,北京100029

出　　处：《基础医学与临床》2011年第5期540-545,共6页Basic and Clinical Medicine

基　　金：北京中日友好医院重点学科建设基金(10035)

摘　　要：目的探讨自身免疫性炎性肌病肺间质病变的病因及发病机制。方法用异种动物骨骼肌匀浆免疫诱导建立自身免疫性炎性肌病大鼠模型,HE染色和Masson染色观察肌肉和肺组织病理改变,免疫组织化学染色结合计算机图像分析方法检测TRAIL及其受体在病变肺组织中的表达。结果 45例自身免疫性炎性肌病大鼠中有16只出现不同程度的肺间质病变(P<0.05);病变肺组织中TRAIL表达由对照组的2 035±657升高至8 934±741(P<0.05),DCR1表达由对照组的6 985±497降低至1 996±401(P<0.05),局部炎性细胞浸润高表达DCR2;肺泡炎性反应程度与肺组织中CD8+T淋巴细胞浸润相关(P<0.01),肺纤维化程度与肺组织中TRAIL表达升高相关(P<0.05)。结论首次证实自身免疫性炎性肌病大鼠模型出现与特发性炎性肌病患者相似肺间质病变,且与局部异常活跃的免疫炎性反应有关,TRAIL及其受体可能参与其中。Objective To investigate the pathogenesis of interstitial lung disease（ILD） in experimental autoimmune myositis（EAM） models.Methods EAM mode was established by injecting the homogenate of rabbit skeletal muscle with Freund＇s complete adjuvant.The histopathological features of the muscle and lung from the EAM model were investigated by HE staining and Masson＇s trichrome.The expression of TRAIL and TRAIL receptors（TRAIL-R1,TRAIL-R2,TRAIL-R3,and TRAIL-R4） in lung tissues was measured by immunohistochemical method and computer image analysis.Results Sixteen of 45 rats with autoimmune inflammatory myopathies had varying degrees of interstitial lung disease（P0.05）;TRAIL expression was increased in lung tissue（2 035±657） and that of DCR1 was decreased（6 985±497）（P0.05）;Inflammatory cells infiltrated locally were found with high expression of DCR2;Alveolitis was related with CD8＋ T lymphocytes（P0.001） and pulmonary fibrosis was related with TRAIL expression in lung tissues（P0.05）.Conclusion The features of ILD shown in EAMs were related with the local immune inflammatory response.The changes in the expression of TRAIL and its receptorsbetween EAMs and controls suggest TRAIL-system probably play a role in the pathogenesis of IIM with ILD.

关 键 词：自身免疫性炎性肌病 肺间质病变 组织病理 TRAIL及其受体 

分 类 号：R593.26[医药卫生—内科学]