中西太平洋鲣卵巢发育特征  

Ovarian development of skipjack(Katsuwonus pelamis)in the Western and Central Pacific Ocean

作　　者：宫领芳[1] 许柳雄[1,2,3] 管卫兵[1,2,3] 朱国平[1,2,3] 李莹春[1] 

机构地区：[1]上海海洋大学海洋科学学院,上海201306 [2]上海海洋大学大洋渔业资源可持续开发省部共建教育部重点实验室,上海201306 [3]上海海洋大学大洋生物资源开发和利用上海市高校重点实验室,上海201306

出　　处：《水产学报》2011年第4期559-568,共10页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2007AA092202);上海市科委重点攻关项目(073205110);上海市重点学科建设项目(S30702)

摘　　要：根据2007年10月-2008年1月在中西太平洋采集的140个卵巢样本,利用组织学分析方法,研究了鲣卵巢的发育过程,详细描述了鲣卵巢、卵细胞的发育阶段。结果表明:(1)组织学上,鲣的卵细胞发育过程分为6个时相,与之对应的,卵巢发育过程分为6个时期。(2)鲣属于不同步产卵类型,卵巢内会同时出现不同时相的卵细胞。(3)传统鉴定方法用于鲣卵巢发育状况时,存在不足之处,与组织学分析的结果差别较大,尤其是Ⅵ期的差别最大。(4)调查海域的鲣至少有两个产卵高峰期,一个高峰期是11月底(12月初),另一个高峰期是12月底(1月初)。It is important to master the knowledge of the ovary development of skipjack,to provide a fundamental information for further study on reproduction mechanism,spawning date and times,batch fecundity,body size of first sex maturation etc.This is also the basic data for the skipjack biological study and resource assessment.140 ovaries of skipjack（Katsuwonus pelamis）have been studied by means of histological method,and the samples were collected from the EEZs of Papua New Guinea and Federal States of Micronesia in the Western and Central Pacific（WCPO）during October 2007 to January 2008 on board purse seine vessels.Samples for histological study were fixed in formalin solution and processed by the standard methods.Sections（thickness 7 μm）were stained by hematoxylin-eosin.In the course of studying ovogenesis,we observed ovary features through Olympus BX-51 microscope camera and determined ovarian development stages.Full stages of maturity and cells progress have been observed and described in detail in this paper reflecting the ovogenesis development.The main results are：（a） Mature development of oocyte can be marked as six periods.According to that,six phases are outlined for ovary.At the first stage,the cell is small and the ratio of nucleolus to cytoplasm is big.At the second stage,a follicular cells layer is around the nucleolus,cytoplasm becomes two layers,there are some small fat drops around the nucleolus.At the third stage,yolk granules occur and increase gradually.At the fourth stage,the cell volume grows bigger while fat drops fuse and yolk granules fuse.At the fifth stage,fertilization hole pouches and ovulation is happening.And at the sixth stage,nucleolus blurs and cytoplasm decreases.（b） Skipjack is a type of multiple spawning species,varied oocyte phases can be observed in one ovary at the same time.GSI changes obviously when ovary develops from stage Ⅲ to Ⅳ and from Ⅴ to Ⅵ,but there exists much overlap from Ⅰ to Ⅵ period.（c） Traditional gonad identification system

关 键 词：鲣 卵巢 中西太平洋 

分 类 号：Q959.4[生物学—动物学] S917[农业科学—水产科学]