内毒素对大鼠肝脏中胞液型磷脂酶A_2表达的影响  

Effects of lipopolysaccharide on expression of cytosolic phospholipase A_2 in hepatic tissues after endotoxemia in rats

作　　者：赵树铭[1] 康格非[2] 华川[1] 雷曼苹[1] 胡珍国[1] 

机构地区：[1]第三军医大学附属西南医院检验科,重庆400038 [2]重庆医科大学医学检验系,重庆400046

出　　处：《第三军医大学学报》1999年第10期753-755,共3页Journal of Third Military Medical University

摘　　要：目的：探讨内毒素血症时胞液型磷脂酶A2（cPLA2）对大鼠肝脏损伤的影响。方法：选用Wistar大鼠30只，分为对照组和内毒素组，分别检测血清常规肝功能生化指标和前列腺素E2（PGE2）的含量，同时观察肝脏组织冰冻切片cPLA2的免疫络化变化。结果：内毒素血症大鼠血清碱性磷酸酶（ALP）和谷氨酸氨基肽酶（GGT）活性及前列腺素E2含量均有升高，肝脏CPLA2表达显著增强。结论；cPLA2可能参与内毒素致肝脏损伤，并介导炎症介质PGE2的产生。Objective: To study the effects of lipopolysaccharide (LPS) on the expression of cytosolic phospholipase A2(cPLA2) in the hepatic tissues during endotoxemia in rats. Methods: 30 Wistar rats were randomized into the control and LPS-treated groups. The indicators of liver functions and the level of prostaglandin E,(PGE2) in the serum of all the experimental animals were determined. The frozen sections of the Iiver tissues, after incubation and immunostaining, were studied with SP method to look for the expression of cPLA2. Results: The activity of ALP and ALT and PGE2 level were higher in the serum of LPS-treated group than in the control and cPLA2 expression was also significantly higher in the LPS-treated group.Conclusion: cPLA, may contribute to elevate the serum level of PGE2 durifig endotoxemia in rats.

关 键 词：肝脏 磷脂酶A 大鼠 内毒素血症 

分 类 号：R515.306[医药卫生—内科学] R575.02[医药卫生—临床医学]