逆转录病毒载体介导的同种MHC基因在小鼠胸腺的表达  

Expression of Allogenic MHC Gene in Mice Thymus by Retroviral Vector

作　　者：李锦华[1] 廖晓星[1] 陈永雄[2] 张仕光[2] 

机构地区：[1]中山医科大学附属第一医院急诊科,广州510080 [2]中山医科大学附属第一医院,肾内科广州510080

出　　处：《现代临床医学生物工程学杂志》1999年第3期180-182,共3页Journal of Modern Clinical Medical Bioengineering

基　　金：国家自然科学基金(No:39570343)

摘　　要：目的:通过胸腺内注射质粒 PXN(N2—B19—H—2K^b),表达外源性主要组织相容性抗原复合物(major histo-compatibility complex,MHC)抗原,为下一步诱导异基因小鼠器官移植耐受作准备.方法:通过 BALB/C 小鼠胸腺内注射质粒 PXN(N2—B19—H—2K^b),将外源性的编码 C57BL/6小鼠MHCI类抗原的H—2K^bcDNA转移到 BALB/C小鼠胸腺,用聚合酶链反应(PCR),反转录聚合酶链反应(RT—PCR),单克隆抗体免疫萤光染色流式细胞仪检测BALB/C小鼠胸腺细胞DNA,mRNA和MHC蛋白质表达,结果:BALB/C小鼠胸腺表面有外源性MHC分子表达,转染效率为5.1%结论:通过胸腺内注射逆转录病毒载体PXN(N2—B19—H—2K^b),小鼠胸腺能表达同种MHC基因,为下一步行异基因小鼠器官移植耐受打下了物质基础.Objective: Exogenous allogenice Major Histocompatibality Complex (MHC) gene was expressed by intrathymic injection of retroviral vector PXN(N2-B19-H-2Kb). Methods: Retroviral vector PXN ( N2 -B19 - H - 2Kb) was injected into BALA/C thymus directly to express MHC of C57BL/6( H - 2b); and the polymerase chain reaction (PCR),the revrse transcription polymerase chain reaction(RT- PCR)and flow cy-tometry assays detected DNA,mRNA and MHC protein expression in thymocytes. Results: Allogenic MHC gene had been integrated into the chromosomal DNA of BALA/C thymus and expressed stably. Conclusion: Expression of alllogenic MHC gene in mice thymus could be achieved by retroviral vector. It should lay the foundation for inducing mice allogenic immunological tolerance .

关 键 词：免疫耐受 移植排斥反应 MHC基因 逆转录病毒 

分 类 号：R392.4[医药卫生—免疫学]