胆固醇分子印迹聚合微球的制备及其吸附性能  

Synthesis of Molecularly Imprinted Polymer Microspheres for Cholesterol and Their Adsorption Property

作　　者：郭永学[1] 礼彤[1] 王立红[1] 吴迪[1] 周丽莉[1] 

机构地区：[1]沈阳药科大学制药工程学院制药工程与环境科学系,辽宁沈阳110016

出　　处：《过程工程学报》2011年第2期318-323,共6页The Chinese Journal of Process Engineering

基　　金：辽宁省教育厅科学研究基金资助项目(编号2009A687)

摘　　要：以胆固醇为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂,在甲苯-乙腈(9:1,Φ)混合溶剂中沉淀聚合制备了分子印迹聚合物微球.采用平衡结合法和Scatchard模型评价了该聚合微球的结合性,考察了其吸附行为.结果表明,胆固醇分子印迹聚合微球中形成两类不同的结合位点,得到高亲和性结合位点的离解常数和最大表观吸附量分别为0.86mmol/L和80.4μmol/g,低亲和性结合位点解离常数和最大表观吸附量分别为0.39mmol/L和61.6μmol/g.此方法合成的分子印迹微球对胆固醇有较好的结合性能,可用于胆固醇的分析检测.Molecularly imprinted polymer microspheres（MIPMs） were prepared by precipitation polymerization of methacrylic acid（MAA） and ethylene dimethacrylate（EDMA） using cholesterol（CHO） as template molecule in toluene-acetonitrile mixed solvent（9：1,Φ）.The MIPMs were investigated in equilibrium binding experiment and Scatchard analysis to evaluate the binding characteristics and recognition mechanism of MIPMs in aqueous solution.Scatchard analysis showed that two classes of binding sites were produced in the polymer matrix under the studied concentration.Their dissociation constant（Kd） and apparent maximum binding capacity（Qmax） were 0.86 mmol/L,and 80.4 μmol/g for high affinity binding sites and 0.39 mmol/L and 61.6 μmol/g for low affinity binding sites.The CHO imprinted polymer micro-spheres synthesized in this method had better performance and could be used for separation,enrichment and analysis of CHO.

关 键 词：胆固醇 分子印迹聚合微球 沉淀聚合 

分 类 号：R917[医药卫生—药物分析学]