荧光定量PCR方法检测脑脊液中结核菌DNA的临床意义  

Clinical Significance of Detection of TB in CSF with Fluorescent Quantitative PCR

作　　者：冯恩航[1] 王冬梅[1] 杨文碧[1] 徐殿霞[1] 

机构地区：[1]黑龙江省医院实验诊断部,黑龙江哈尔滨150036

出　　处：《黑龙江医学》2011年第4期281-283,共3页Heilongjiang Medical Journal

摘　　要：目的探讨应用荧光定量PCR方法检测脑脊液中结核菌DNA(TB-DNA)对结核性脑膜炎的临床意义。方法采用荧光定量PCR方法对68例结核性脑膜炎患者及32例非结核性脑膜炎患者的脑脊液标本进行检测,并与涂片、培养法比较。结果荧光定量PCR方法的敏感性显著高于涂片及培养,阳性率分别为57.4%、2.9%、8.9%(P<0.001)。结论荧光定量PCR方法可以通过标准品制定标准曲线,根据标准曲线可换算出标本中原始的靶DNA值,同时具有较高的特异性和敏感性,对结核性脑膜炎的诊断具有重要的临床指导意义。Objective To discuss fluorescence quantitative PCR method of cerebrospinal fluid in tuberculosis DNA（TB-DNA） on the clinical significance of tuberculous meningitis.Methods The quantitative PCR method in 68 cases of tuberculous meningitis and 32 cases of non-tuberculous meningitis in cerebrospinal fluid specimens of patients detected with smear,culture comparison.Results Fluorescence quantitative PCR method was significantly higher than the sensitivity of smear and culture positive rate was 57.4%,2.9%,8.9%（P0.001）.Conclusion The quantitative PCR method could be developed through the standard calibration curve,according to the standard curve could be converted from the original specimens of the value of the target DNA,but also have high specificity and sensitivity of the diagnosis of tuberculous meningitis have important clinical guidance significance.

关 键 词：结核分支杆菌-DNA 荧光定量PCR法 脑脊液 

分 类 号：R446.61[医药卫生—诊断学]