成骨细胞复合β-磷酸三钙异位成骨的实验研究  

Osteogenesis of The Construct Combined Osteoblasts With β-TCP in Rat

作　　者：张明宇[1] 郑江[1] 杨镇[1] 张宪[1] 刘阳[1] 周伟[1] 周运平[1] 

机构地区：[1]西安市红十字会医院运动损伤科,710054

出　　处：《美中国际创伤杂志》2011年第1期15-17,共3页U.S.Chinese International Journal of Traumatology

摘　　要：目的：评价成骨细胞复合多孔β-磷酸三钙支架在大鼠肌肉中的成骨效果。方法：分别在植入1、4、8周后，将成骨细胞β-磷酸三钙复合物取出行HE染色观察新骨形成情况，并应用KS4003．0软件进行新骨生成分析。结果：植入1周后所有标本都未发现骨生成；在4周后实验组新骨生成量为6．35％，对照组为1．32％：8周后实验组新骨生成晕为21．58％，对照组新骨生成量为4．78％。两组比较有显著性差异。结论：成骨细胞复合多孔β-磷酸二钙支架可以促进新骨生成。Objective： To evaluate the ossification result of construct combined osteoblasts with β-triealeium phosphate （β-TCP）as bone substitute implanted in rat dorsal muscles. Methods： Specimens were implanted and took out on 1 week, 4 and 8 weeks after implantation as well as observed by hematoxylin and eosin （HE）staining. The percentages of new bone formation for each group and implantation period were determined by histomorphometry. Results： After 1 week＇s implantation, new bone formation in both β-TCP and osteoblasts＋β-TCP groups did not noted. At 4 weeks, the amount of bone formation was increased to 1.32 % in β-TCP group and 6.35% in osteoblasts＋β-TCP group. 8 weeks later, more bone was found in the osteoblasts＋β-TCP group （21.58 %）, while in the β-TCP group bone formation was increased to 4.78%, there was a significant difference between two groups. Conclusion： Based on these results, we concluded that bone substitutes constructed by porous β-TCP scaffold loaded with osteoblasts can promote new bone formation.

关 键 词：成骨细胞 β-磷酸三钙(β-TCP) 骨生成 

分 类 号：R318[医药卫生—生物医学工程]