束缚-浸水应激对大鼠迷走背核胆碱能神经元活动的影响  

Influence of restraint water-immersion stress on activity of cholinergic neurons in the dorsal motor nucleus of the vagus in the rat

作　　者：赵东芹[1] 艾洪滨[1] 

机构地区：[1]山东师范大学生命科学学院生理学教研室,济南250014

出　　处：《山东大学学报（医学版）》2011年第3期13-17,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30770277;30970354;31071920)

摘　　要：目的研究束缚-浸水应激(RWIS)大鼠迷走神经背核(DMV)中胆碱能神经元的活动情况。方法随机将10只雄性Wistar大鼠分为对照组和应激组,采用Fos和胆碱乙酰化酶(ChAT)免疫组织化学双标技术,统计对照组和应激组Fos、ChAT、Fos/ChAT双标阳性神经元数目。结果与对照组相比,应激组大鼠DMV大量神经元表达Fos,Fos阳性神经元主要集中于DMV尾段和吻段(P<0.01);ChAT阳性神经元主要分布于DMV中段和尾段,应激组大鼠DMV中单位面积内ChAT阳性神经元数目减少(P<0.01);Fos/ChAT双标阳性神经元的分布与Fos阳性神经元分布情况相似,RWIS组Fos/ChAT双标阳性神经元数目显著增加(P<0.01),Fos/ChAT双标阳性神经元占ChAT阳性神经元的比例在对照组和RWIS组中分别为7.17%、21.12%(P<0.01)。结论 DMV中胆碱能神经元参与RWIS调控过程。Objective To investigate activity of cholinergic neurons in the dorsal motor nucleus of the vagus（DMV） in response to water-immersion stress（RWIS） in rats.Methods Ten rats were randomly divided into the control group and the RWIS group.Fos-immunoreactive（Fos-IR）,choline acetyltransferase immunoreactive（ChAT-IR） and double labeled（Fos/ ChAT-IR） neurons were counted using dual Fos and ChAT immunohistochemistry.Results Compared with unstressed rats,Fos-IR neurons dramatically increased in the DMV of RWIS rats（P0.01）,and Fos expression was higher in the caudal portion of the NTS compared with the rostral and intermedial portions.ChAT-IR neurons decreased and were mainly observed in the caudal and intermediate portions of the DMV in RWIS rats（P0.01）.Percentages of Fos/ ChAT-IR norons in ChAT-IR neurons in unstressed and RWIS rats were 7.17% and 21.12%,respectively（P0.01）.Conclusion Cholinergic neurons in DMV are involved in the stress response.

关 键 词：应激 迷走神经背核 胆碱O-乙酰转移酶 FOS 大鼠 

分 类 号：Q42[生物学—神经生物学]