脂肪间充质干细胞移植对兔心梗后近期心功能的影响  

Effect of transplantation of adipose tissue-derived mesenchymal stem cells on short-term heart function in rabbits with myocardial infarction

作　　者：张卫泽[1] 肖立运[2] 陈永清[1] 马凌[1] 褚嫒嫒[2] 

机构地区：[1]兰州军区兰州总医院心血管内科,甘肃兰州730050 [2]兰州大学第二临床医学院,甘肃兰州730000

出　　处：《心脏杂志》2011年第2期193-196,共4页Chinese Heart Journal

基　　金：全军医药卫生科研基金资助项目(06MB094)

摘　　要：目的:观察脂肪间充质干细胞(ADMSCs)心肌内移植对兔心肌梗死(MI)后心力衰竭近期心功能的影响,并初步探讨其机制。方法:将30只兔随机分为假手术组(只穿线不接扎)、对照组(心肌内注射IMDM培养基)及干细胞移植组(心肌内注射ADMSCs),每组10只(n=10);结扎兔冠状动脉左前降支(LAD)制作急性MI模型,同时将6-二氨基-乙-苯茚二酮(DAPI)标记的ADMSCs注射到梗死区数个不同部位。造模前及移植后4周、8周,用超声心动图检测心功能,术后8周处死动物测量左室体质量指数及组织学观察,采用免疫荧光方法鉴定移植的细胞。结果:移植后4周起,心功能指标明显改善(P<0.05),术后第8周心功能指标改善更明显(P<0.01),左室重构程度也得到明显改善(P<0.01)。结论:移植的ADMSCs可在宿主体内成活,并显著改善兔MI后的心功能。AIM： To study the effect of transplantation of adipose tissue-derived mesenchymal stem cells（ADMSCs） on short-term heart function in rabbits with myocardial infarction（MI）.METHODS： A total of 30 Japanese big ear albino rabbits were selected and randomly divided into three equal groups： sham-operation group（A）,control group（B）,ADMSCs transplantation group（C）.MI models were established in rabbits in B and C groups.The left anterior descending（LAD） arteries of the rabbits were ligated to establish animal models of acute MI（AMI） and DAPI-marked ADMSCs were transplanted into the models.Before transplantation and 4 and 8 weeks after transplantation,heart functions were examined by ultrasonographic cardiogram test and left ventricular weight index was measured.RESULTS： Heart function improved 4 weeks after transplantation and the left ventricular weight index reduced significantly 8 weeks after transplantation.CONCLUSION： Transplanted ADMSCs can survive and improve heart functions in rabbits with heart failure after AMI.

关 键 词：脂肪间充质干细胞 移植 左室重构 兔 

分 类 号：R542.22[医药卫生—心血管疾病]