PCL/SS纳米纤维支架的制备及相容性研究  

Preparation and Cytocompatibility Study of Poly (ε-caprolactone)/Silk Sericin Nanofibrous Scaffolds

作　　者：李海滨[1] 李林昊[1] 钱宇娜[1] 蔡开勇[1] 吕永钢[1] 钟莉[1] 刘万钱[1] 杨力[1] 

机构地区：[1]重庆大学生物工程学院生物流变科学与技术教育部重点实验室,重庆400044

出　　处：《生物医学工程学杂志》2011年第2期305-309,共5页Journal of Biomedical Engineering

基　　金：国家重点基础研究发展计划项目资助(2005CB522703);国家“111计划”项目资助(B06023);国家自然科学基金资助项目(30870608);重庆市科技攻关重大项目资助(2009AA5045)

摘　　要：采用静电纺丝技术制备了聚己内酯/丝胶蛋白(PCL/SS)纳米三维多孔支架,利用扫描电镜(SEM)、透射电镜(TEM)、傅立叶红外光谱(FTIR)技术、接触角仪等手段对其结构和性能进行了表征。取人皮肤成纤维细胞(FEK4)与支架复合培养,SEM和MTS法观察了细胞在材料上的黏附与增殖情况。结果表明,所制备的含SS纳米颗粒的PCL/SS纳米纤维支架呈相互交联的多孔网状无纺结构,纤维直径均一度与精细度高,且其中SS二级结构没有发生变化。SS加入后改善了材料表面的亲水性,增强FEK4细胞在材料上的黏附及增殖。Three-dimensional poly（ε-caprolactone）/silk sericin（PCL/SS） porous nanofibrous scaffolds were prepared by electrospinning.The structure and properties of the scaffolds were characterized by Scanning Electron Microscope（SEM）,Transmission Electron Microscope（TEM）,Fourier Transform Infrared Spectroscopy（FTIR） and water contact angle instrument.Studies on cell adhension and proliferation were carried out by culturing human primary skin fibroblast cells（FEK4） on these scaffolds using SEM and MTS.The experimental results showed that the PCL/SS nanofibrous scaffolds with SS nanoparticles had porous non-woven mesh structure with nanofibrous cross-linked with each other.Fiber diameter was very uniform and precise,and the secondary structure of SS protein had not been changed.Furthermore,the capability of hydrophile increased with the SS addition,which improved FEK4 cells adhesion and proliferation on the scaffolds.

关 键 词：静电纺丝 聚己内酯 丝胶蛋白 纳米颗粒 

分 类 号：R318.08[医药卫生—生物医学工程]