聚乳酸-O-羧甲基壳聚糖纳米粒子对肝细胞生长因子的载药和体外释药行为研究  

PLA-O-CMC Nanoparticles: HGF Loading and Delivery Behaviors in Vitro

作　　者：李志峰[1] 陈钟[2] 常仁安[2] 

机构地区：[1]南通大学附属南通市妇幼保健院外科,南通226006 [2]南通大学附属医院肝胆外科,南通226001

出　　处：《生物医学工程学杂志》2011年第2期314-317,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30772130);江苏省"六大人才高峰"项目资助(07-B-018)

摘　　要：研究聚乳酸-O-羧甲基壳聚糖纳米粒子(PLA-O-CMC NPs)对肝细胞生长因子(HGF)的载药能力,探讨载HGF的PLA-O-CMC NPs体外释药行为。以PLA和O-CMC为基质材料制备载HGF的PLA-O-CMC NPs,并观察其形态;计算药物包封率,分析PLA-O-CMC NPs对HGF的载药能力;研究载HGF的PLA-O-CMC NPs短期和长期释药能力。载HGF的PLA-O-CMC NPs呈球形,平均粒径为139.82 nm,最大包封率为74.2%。该载药纳米粒子体外HGF的累积释放量在前24 h内逐步上升,并有明显的突释现象,释放出的药物量占释放总量的36.7%。从第2 d开始,HGF的释放量明显下降,但在较长时间内持续稳定释放。PLA-O-CMC NPs是一种良好的HGF载体。载HGF的PLA-O-CMC NPs在体外能够迅速释放HGF,达到有效药物浓度,并能够在较长时间内维持一定的有效药物浓度。This paper is aimed to observe the hepatocyte growth factor（HGF） loading and delivery ability of polylactic acid and oxygen carboxymethylated chitosan copolyer nanoparticles（PLA-O-CMC NPs）.We prepared PLA-O-CMC NPs loaded with HGF by ultrasound in combination with magnetic stirring method.The NPs were characterized by transmission electron microscopy,embedding ratio;drug loading and drug delivery behaviors were observed by ELISA.The characteristics of PLA-O-CMC NPs loaded with HGF showed that the mean size was 139.82 nm,polydispersity was 0.108,maximal HGF-embedding ratio was 76.32%.The cumulative HGF release gradually increased in the first 24 hours in vitro,with sharp increasing in the first 7 hours,and moderate and steady increasing in the following 17 hours.The HGF had a burst release in the first 24 hours,and in this process the released HGF took up 36.7% of the whole release.From the second day,the HGF release decreased obviously,while it kept on releasing steadily（45～55 ng/d） for quite long time up to 30 days.The experiment proved that PLA-O-CMC NPs is a favourable carrier of HGF.PLA-O-CMC NPs loaded with HGF could rapidly release HGF in vitro.The released HGF reached the effective drug concentration and maintained the certain effective drug concentration for a long time.

关 键 词：PLA-O-CMC纳米粒子 肝细胞生长因子 载药 释药 

分 类 号：R318.08[医药卫生—生物医学工程]