体外培养神经干细胞NGF、BDNF和NT-3及其受体trkA、trkB和trkC的表达  

Expression of NGF,BDNF,NT-3 and Their Receptors trkA,trkB,trkC in Cultured Neural Stem Cells

作　　者：李玉[1] 王波[1] 林娜[2] 王廷华[2] 习杨彦彬[2] 

机构地区：[1]昆明医学院第一附属医院神经外科,云南昆明650032 [2]昆明医学院神经科学研究所,云南昆明650031

出　　处：《昆明医学院学报》2011年第2期3-6,共4页Journal of Kunming Medical College

基　　金：国家自然科学基金资助项目(30260125)

摘　　要：目的检测体外培养神经干细胞(NSC)神经生长因子(NGF),脑源性深营养因子(BDNF),神经营养因子3(NT-3)及其受体trkA、trkB、trkC的表达.方法胎鼠海马神经干细胞培养并鉴定后,提取其总RNA,RT-PCR技术检测NGF、BDNF、NT-3及其受体trkA、trkB、trkC的表达情况.免疫组化染色观察三种生长因子在NSC的分布和亚细胞定位.结果 RT-PCR显示NSC表达三种生长因子及其受体mRNA.免疫组化染色显示NSC表达三种生长因子蛋白,免疫阳性产物主要分布于细胞质.结论体外培养NSC表达NGF,BDNF,NT-3及其受体,提示三种因子对NSC发育和发挥生物学功能可能有重要作用.Objective To investigate the expression of NGF, BDNF, NT 3 and their receptors trkA, trkB, trkC in cultured Neural stem cells （NSCs）. Methods NSCs were isolated from embryonic hippocampus of mice. They were then cultured in vitro and identified by immunohistochemistry using nestin antibody. The expression of mRNA and protein for NGF, BDNF, NT 3 was detected by RT PCR and immunohistochemistry. Results Significant levels of mRNA were detected for all three factors and their receptor trkA, trkB, trkC by RT PCR. The proteins of three factors and their receptors were located in cytoplasma found by immunohistochemistry. Conclusion Cultured NSCs express NGF, BDNF, NT 3, and their receptors, suggesting that the three factors play an important role in NSCs development and function maintainance.

关 键 词：小鼠 神经干细胞 神经营养因子 基因表达 

分 类 号：R344.13[医药卫生—基础医学]