马尔尼菲青霉菌溶血磷脂酶基因的识别和序列分析及功能预测  

Identification and sequence analysis and functional prediction of the gene encoding lysophospholipase from Penicillium marneffi

作　　者：李凌华[1] 胡凤玉[1] 陈万山[1] 宋伟南[1] 蔡卫平[1] 唐小平[1] 

机构地区：[1]广州医学院附属市八人民医院感染病科,510060

出　　处：《国际流行病学传染病学杂志》2011年第2期73-77,共5页International Journal of Epidemiology and Infectious Disease

基　　金：国家十一五科技重大专项课题（2008ZX10001-008）;广东省自然科学基金（10151006002000000）;广州市卫生局医药卫生科技重点项目（2009-ZDi-016）

摘　　要：目的 从马尔尼菲青霉菌（PM）酵母相全长cDNA文库中识别溶血磷脂酶基因，预测其结构和功能，为进一步实验研究提供理论依据。方法利用NCBI在线分析工具对PM酵母相全长cDNA文库进行筛选，识别溶血磷脂酶基因；通过VectorNTIsuite8．0软件包，对其编码蛋白质的各种结构与功能特征进行预测，并构建种系分子进化树。结果筛选得到的基因长1014bp，Blastx分析该基因可能是编码PM溶血磷脂酶的全长基因，完整的开放读码框（ORF）共含729bp，编码243个氨基酸；其编码蛋白的相对分子质量为26800，预测等电点为5．49，疏水氨基酸占47．7％，亲水氨基酸占26．8％，酸性氨基酸占12．7％，碱性氨基酸占12．8％；该蛋白有潜在的2个酪蛋白激酶Ⅱ磷酸化位点、3个蛋白激酶C磷酸化位点和7个N-肉豆蔻酰位点。结论该氨基酸序列属于溶血磷脂酶样功能域特征蛋白酶；与球孢子菌亲源关系最近。通过研究，一个PM溶血磷脂酶基因被成功发现，这为进一步探讨该基因的功能提供了条件。Objective To identify the gene encoding lysophospholipase from the full length cDNA library of Penicillium marneffei （PM） in yeast phase and predict the structure and function of its deduced protein, to provide with theoretical evidences for further experiments. Methods The PM full-length cDNA library in yeast phase was screened to identify the gene encoding lysophospholipase with the help of NCBI on-line analytical tool. Then, by utilizing the software package of Vector NTI suite 8.0, the protein deduced by the gene was analyzed to predict its corresponding structure and functions, and its molecular cladogram was constructed. Results The gene was composed of 1014 base pairs in the length and was presumed to be the full-length gene encoding lysophospholipase by Blastx, with a complete open reading frame （ORF） comprised of 729 base pairs encoding 243 amino acids with relative molecular weight being 26 800 and the predicted isoelectric point being 5.49. The deduced protein included 47.7% hydrophobic amino acids, 26.8% hydrophilie amino acids, 12.7% acid amino acids and 12.8% basic amino acids. The protein had 2 potential casein kinase 11 phosphorylation site, 3 potential protein kinase C phosphorylation site and 7 N-myristoyl site. Conclusions The amino acid sequence belongs to this kind of protease with lysophospholipase-like domain. The protein is most close to Coccidioides posadasii in genetic relationship. A novel gene encoding lysophospholipase is successfully found and the work has made necessary preparations for further research on the gene＇ s function.

关 键 词：溶血磷脂酶 马尔尼菲青霉菌 生物信息学 

分 类 号：R378[医药卫生—病原生物学]