布鲁杆菌M16株pgm基因突变活菌苗株毒力初步评价  

Preliminary virulence evaluation on mutation of pgm attenuates Brucella melitensis 16 strain

作　　者：李鹏[1] 潘玉焕[1] 罗德炎[2] 王希良[2] 

机构地区：[1]空军航空医学研究所附属医院,北京100089 [2]军事医学科学院微生物流行病研究所免疫研究室

出　　处：《中国地方病防治》2011年第2期84-87,共4页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然基金(№.30170853)

摘　　要：目的通过减毒性实验评价布鲁杆菌M16株pgm基因突变活菌苗株毒力减弱情况,为新型减毒活疫苗的研制和布鲁杆菌感染免疫防御的研究奠定基础。方法进行布鲁杆菌侵袭实验等动物和细胞实验评价突变菌株的毒力减弱情况。结果突变菌株在小鼠体内能生存,但较强毒株存活数量有很大减少(P<0.001);突变菌株较强毒株对多粘菌素B更为敏感,存活率与强毒株相比有明显下降(P<0.001);突变菌株LPS较强毒株LPS明显缺失;突变菌株在体外He-La细胞中能存活但复制减弱。结论布鲁杆菌M16株pgm基因突变菌株毒力减弱。可作为未来布鲁杆菌新型减毒活疫苗的候选者,有进一步研究的价值。Objective Evaluate the effect of attenuation and lay the foundation for the research of immunological mechanism of brucella infection and the development of novel live attenuated vaccines.Methods The effect of attenuation is evaluated by brucella invasion experiment,and other animal and cell experiments.Results Compared with wild-type strains,the mutation strains have several aspects different from it ：（1） though the mutation strains of Brucella melitensis 16.can survive in the mouse model,the survival rates in the spleens of the inoculated mice were significantly lower than the wild-type strain;（2） the mutation strains were more sensitive to PmB than the wild-type parental strain;（3） the amount of LPS on the mutation strain of Brucella melitensis 16.were seen less than on the wild-type parental strain;（4） the replication rate of mutatation stains in HeLa cell line decreased dramatically although it can survive in it in vitro.Conclusions We have confirmed the attenuated effect of the mutation strain of Brucella melitensis 16,it can be used as a candidate vaccine for the control of brucellosis.

关 键 词：布鲁杆菌 减毒活疫苗 毒力 

分 类 号：R516.7[医药卫生—内科学]