UPT技术在人间鼠疫疫情处理中的展望  

Prospects of UPT technology for in the treatment of human plague epidemic

作　　者：张宏[1] 达文平[1] 苗克军[1] 郭丽民[1] 葛亚俊[1] 徐大琴[1] 潘卫民[1] 席进孝[1] 

机构地区：[1]甘肃省疾病预防控制中心,甘肃兰州730020

出　　处：《中国地方病防治》2011年第2期122-123,共2页Chinese Journal of Control of Endemic Diseases

摘　　要：目的通过分析UPT技术检测人间鼠疫疫情材料结果,探索该技术在人间疫情中的应用前景。方法应用ELISA、金标(、R)IHA、细菌培养和UPT方法检测人间鼠疫疫情材料,统计分析UPT技术在检测人间鼠疫疫情中灵敏度和特异度。结果用ELISAI、HA、UPT、金标法检测血清55份,χ2=39.808,阳性率差异具有统计学意义。UPT阳性率最高,敏感为100%,特异度为59.0%,ROC曲线值高于其他方法。检测6份鼠疫特异性F1抗原结果一致。结论 UPT检测技术灵敏度高、特异性较强,ROC曲线面积值最大说明检测结果最接近真值,是筛检试验的首选方法。将该检测技术应用鼠疫疫情材料检验,能充分发挥该技术操作便捷、快速检测的作用。特别是在检验材料量大时,用该技术进行筛检后,只对其检测阳性材料进行其它方法检验,可以节省大量人力、财力,也可对疫情确定和发现感染者起到提示性作用。Objective To analyse the result of the epidemic materials from human plague detected by the methods of UPT,and to explore the application prospect in human plague.Methods The epidemic materials of human plague were tested by the methods of GICA,ELISA,IHA,bacterium culture and UPT.Then the sensitivity and specificity of UPT on the detection of human plague was analysed.Results 55 portions of serum were checkouted by the methods of GICA,ELISA,IHA and UPT,which the value of χ2 is 39.808.It was showed that the positive rate are statistically significant differences.The positive rate through the methods of UPT was the highest,the sensitivity of it was 100%,the specificity of it was 59.0%,the curves of ROC was higher than other methods.The result remain identical through testing 6 portions of F1 Antigen.Conclusions Because of the high sensitivity and stronger specificity of UPT,the maximum area of ROC curves showing close to true value,UPT was the preferred method on screening tests.The application of UPT into test epidemic materials from human plague can fully exert convenient operation and fast detection.Especially in the large amount of epidemic materials,after the screening,the positive temple were detected by other methods.It can save you a lot of labour and finance,determine the human plague find out the infected patients.

关 键 词：上转换发光技术(UPT) 鼠疫疫情 展望 

分 类 号：R516.8[医药卫生—内科学]