氟伐他汀对内脏脂肪素诱导的内皮细胞MMP-1、TIMP-1和ICAM-1表达的影响  

Effects of Fluvastatin on the Expression of Matrix Metalloproteinase-1,Tissue Inhibitors of Matrix Metalloproteinase-1 and Intracellular Adhesion Molecule-1 Induced by Visfatin in Cultured Human Umbilical Vein Endothelial Cells

作　　者：高歌[1] 郑丽丽[1] 杨静[1] 李志臻[1] 叶启霞 华海婴 

机构地区：[1]郑州大学第一附属医院 [2]河南省医药科学研究院,河南省郑州市450052

出　　处：《中国动脉硬化杂志》2011年第2期121-124,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的观察氟伐他汀对内脏脂肪素诱导的人脐静脉内皮细胞基质金属蛋白酶1、组织型金属蛋白酶抑制剂1、细胞间黏附分子1表达的影响,探讨他汀类药物降脂外抗动脉粥样硬化的作用机制。方法体外培养人脐静脉内皮细胞,以不同浓度氟伐他汀和内脏脂肪素共同孵育24 h,用逆转录聚合酶链反应法检测基质金属蛋白酶1、组织型金属蛋白酶抑制剂1和细胞间黏附分子1的表达。结果与正常对照组比较,内脏脂肪素组基质金属蛋白酶1和细胞间黏附分子1的表达显著升高,组织型金属蛋白酶抑制剂1的表达量下降。氟伐他汀干预后,基质金属蛋白酶1和细胞间黏附分子1的表达较内脏脂肪素组显著降低,组织型金属蛋白酶抑制剂1的表达增加。结论氟伐他汀可能通过抑制内脏脂肪素的活性,影响血管内皮细胞基质金属蛋白酶1、组织型金属蛋白酶抑制剂1和细胞间黏附分子1的表达,由此稳定血管内皮环境,抑制内皮细胞炎症反应,起到抗动脉粥样硬化的作用。Aim To observe the effect of fluvastatin on the expression of matrix metalloproteinase-1（MMP-1）,tissue inhibitors of matrix metalloproteinase-1（TIMP-1）,and intracellular adhesion molecule-1（ICAM-1） induced by visfatin in cultured human umbilical vein endothelial cells（HUVEC）,and to explore the effect and mechanism of anti-inflammatory of fluvastatin. Methods HUVEC were cultured in vitro,the HUVEC were pretreated with fluvastatin at different concentrations（10-7 mol/L,10-6 mol/L,10-5 mol/L） for 20 minutes before incubated with the visfatin of 800 μg/L for 24 hours.The cellular total RNA were extracted by TRNzol reagent.The expression of MMP-1,TIMP-1 and ICAM-1 mRNA were detected by reverse transcription-polymerase chain reaction（RT-PCR）.The results of gel electrophoresis were analyzed with a computer scanning system. Results Different concentrations of fluvastatin could inhibit the expression of MMP-1 mRNA and ICAM-1 mRNA induced by visfatin in a dose-dependent manner.Different concentrations of fluvastatin could up-regulate the expression of TIMP-1 mRNA induced by visfatin. Conclusion Fluvastatin could inhibit inflammatory response in vascular endothelial cells induced by visfatin in a dose-dependent manner,which could protect endothelial cells against the functional disorder,and prevent the development of atherosclerosis.

关 键 词：氟伐他汀 内脏脂肪素 动脉粥样硬化 基质金属蛋白酶1 组织型金属蛋白酶抑制剂1 细 

分 类 号：R96[医药卫生—药理学]