菟丝子黄酮对缺血再灌注大鼠心肌细胞凋亡的影响  

Effect of Flavonoids from Cuscuta Chinensis on Myocardial Cells Apoptosis in Ischemic/Reperfused Rat Hearts

作　　者：翟宏颖[1] 王桂敏[1] 

机构地区：[1]辽宁医学院附属第一医院中医科,辽宁省锦州市121001

出　　处：《中国动脉硬化杂志》2011年第3期211-215,共5页Chinese Journal of Arteriosclerosis

摘　　要：目的观察菟丝子黄酮对缺血再灌注大鼠心肌细胞凋亡及相关基因表达的影响。方法结扎大鼠冠状动脉左前降支30 min,再灌注2 h建立心肌缺血再灌注模型。将50只SD大鼠随机分为假手术组、缺血再灌注组及菟丝子黄酮低、高剂量组和消心痛组。再灌注结束后检测血清肌酸激酶和乳酸脱氢酶,DNA末端标记法计算凋亡指数,免疫组织化学法和Western Blotting法检测心肌Bcl-2和Bax蛋白表达,计算Bcl-2/Bax值。结果与假手术组比较,缺血再灌注组心肌酶肌酸激酶、乳酸脱氢酶和凋亡指数显著增高(P<0.01),Bcl-2和Bax蛋白表达增强(P<0.01);与缺血再灌注组比较,菟丝子黄酮低、高剂量组及消心痛组能降低血清肌酸激酶、乳酸脱氢酶含量和凋亡指数(P<0.01),增加Bcl-2而减少Bax的表达(P<0.01)。结论菟丝子黄酮能够抑制缺血再灌注损伤大鼠的心肌细胞凋亡,与消心痛效果相近。Aim To observe the effect of flavonoids from Cuscuta Chinensis on myocardial cell apoptosis and the related gene expression in ischemic/reperfused myocardial infarction. Methods The left anterior descended coronary artery was ligated for 30 min and reperfused for 2 h to make ischemia-reperfusion injury model.50 SD rats were randomly divided into the sham operation group,ischemia-reperfusion group,the low and high dose groups of flavonoids from Cuscuta Chinensis and isosorbide dinitrate group.Creatine kinase（CK） and lactate dehydrogenase（LDH） were assayed.The apoptotic index（AI） was analyzed by TUNEL staining.The protein expression of Bcl-2 and Bax were measured by immunohistochemistry and Western Blotting in each group,the ratios of Bcl-2 and Bax were calculated. Results Compared with the sham operation group,the ischemia-reperfusion group of enzymes CK and LDH,AI,and Bcl-2 and Bax protein expression were significantly higher（P0.01）;The enzyme CK and LDH,and AI in the low and high dose group of flavonoids from Cuscuta Chinensis were decreased compared with those of the ischemia-reperfusion group（P0.01）.The expression of Bcl-2 was increased and the expression of Bax was decreased（P0.01）.The isosorbide dinitrate group showed no significant difference when compared with the high dose group of flavonoids from Cuscuta Chinensis（P0.05）. Conclusion The pretreatment of flavonoids from Cuscuta Chinensis could dose-dependently decrease enzymes CK,LDH levels,increase the expression of Bcl-2 protein and Bcl-2/Bax,reduce the expression of Bax protein,and inhibit the apoptosis of myocardial cell.The therapeutic effects was similar to isosorbide dinitrate.

关 键 词：菟丝子黄酮 缺血再灌注损伤 细胞凋亡 BCL-2蛋白 BAX蛋白 

分 类 号：R96[医药卫生—药理学]