单核细胞趋化蛋白1在重症急性胰腺炎相关肺损伤中的作用  

Role of monocyte chemotactic protein-1 gene in acute lung injury during acute necrotizing pancreatitis

作　　者：许永春[1] 李兆申[2] 龚燕芳[2] 金晶[2] 

机构地区：[1]南昌解放军第九四医院消化内科 [2]第二军医大学附属长海医院消化科,上海200433

出　　处：《中华胰腺病杂志》2011年第2期123-126,共4页Chinese Journal of Pancreatology

基　　金：全军医药卫生科研基金资助（08MA064）

摘　　要：目的 探讨单核细胞趋化蛋白1（MCP-1）在急性坏死性胰腺炎（ANP）早期并发急性肺损伤（ALI）发病机制中的作用.方法 按数字表法将40只SD大鼠随机分为对照组和ANP 3、6、12 h组,每组10只.采用15%左旋盐酸精氨酸2.0 mg/g体重腹腔注射方法制作大鼠ANP模型,观察胰腺及肺组织病理学改变,检测肺湿/干重比,RT-PCR检测肺组织MCP-1 mRNA的表达.结果 腹腔注射左旋盐酸精氨酸后大鼠胰腺发生出血、坏死,符合ANP病理改变.ANP组肺组织明显水肿,3、6、12 h的病理评分分别为3.75±0.58、5.50±0.63、5.86±0.54;肺湿/干重比为4.85±0.38、4.97±0.47、5.03±0.46;肺组织MCP-1 mRNA表达量为0.36±0.08、0.56±0.15、0.72±0.21.均较对照组的0.12±0.05、4.32±0.33、0.21±0.05显著升高（P＜0.05或＜0.01）,且MCP-1 mRNA表达与肺湿/干重比及肺组织损害程度均呈正相关（r=0.75,r=0.89,P＜0.05）.结论 ANP早期肺组织MCP-1 mRNA表达上调,并在ANP并发的ALI中发挥重要作用.Objective To explore the potential role of monocyte chemotactic protein-1 （ MCP-1 ） gene in the pathogenesis of acute lung injury （ALI） in early acute necrotizing pancreatitis （ANP）. Methods Forty SD rats were randomly divided into control group, ANP 3, 6, 12 h group with 10 rats in each group according to a number table. ANP was induced by intraperitoneal injection of 15% L-arginine solution at a dose of 2.0 mg/g body weight. Pathological changes of pancreases and lungs were observed. Lung wet/dry weight ratio was measured. Intrapulmonary expression of MCP-1 mRNA was evaluated by RT-PCR. Results After intraperitoneal injection of 15% L-arginine solution, the rat＇s pancreas presented with bleeding, necrosis comparable with pathological changes of ANP. Pulmonary tissue edema was obvious. At ANP 3, 6, 12 h group, the pathological scores of the lung were 3.75±0.58,5.50 ±0.63,5.86 ±0.54, the wet/dry weight ratios were 4.85 ±0.38,4.97 ± 0.47,5.03 ± 0. 46, the MCP-1 mRNA expressions were 0.36 ± 0.08, 0. 56 ± 0. 15, 0. 72 ± 0.21,which were significantly higher than those in the control group （0.12 ±0.05,4.32 ±0.33,0.21 ±0.05, P＜0.05 or ＜0.01 ）. The MCP-1 mRNA expression in lungs was significantly correlated with the degree of lung damage and wet/dry weight ratio of lungs （r=0.75,r=0.89,P＜0.05）.Conclusions MCP-1 mRNA expression was up-regulated in the early phase of ANP in the lungs, and it may play an important role in ALI during ANP.

关 键 词：胰腺炎 急性坏死性 单核细胞趋化蛋白1 肺组织 基因表达 

分 类 号：R657.51[医药卫生—外科学]