力学刺激和成骨化学诱导剂对大鼠骨髓间充质干细胞成骨分化能力的影响  

Effects from mechanical stimulation and osteogenic chemical inductor on osteoblastic differentiation of rat bone mesenchymal stem cells

作　　者：何学令[1,2] 姚晓玲[1] 冯贤[1] 邱飞远[1] 李凯[1] 吴江[1] 李良[1] 

机构地区：[1]四川大学基础医学与法医学院,生物医学工程研究室,成都610041 [2]四川大学实验动物中心,成都610041

出　　处：《医用生物力学》2011年第2期116-120,共5页Journal of Medical Biomechanics

基　　金：国家自然科学基金资助项目(30770534,10972149)

摘　　要：目的探讨力学刺激与成骨化学诱导剂对大鼠骨髓间充质干细胞(rat bone mesenchymal stem cellsr,BMSCs)碱性磷酸酶(alkaline phosphatase,ALP)I、型胶原(collagen type I,COL I)和骨钙素(osteocalcin,OCN)基因表达与钙化结节形成的影响。方法体外分离培养rBMSCs,分别在成骨诱导和非成骨诱导条件下应用双轴力学应变加载系统对rBMSCs施加周期性的机械张应变(应变2%,频率1 Hz,每次2 h,间隔2 h,每天加载3次)。力学刺激作用3 d和6 d,采用实时荧光定量RT-PCR检测ALP、COL I和OCN的mRNA表达,同时采用茜素红染色法观察钙化结节的形成情况。结果力学刺激作用后,成骨诱导6 d组出现明显的钙化结节,其余各组均无明显钙化结节形成。与相应非诱导组比较,成骨诱导3 d组ALP、COL I和OCN的mRNA表达量分别增加0.63、和11.8倍;成骨诱导6 d组ALP、COL I和OCN的mRNA表达量分别增加2.73、.2和10倍。结论成骨化学诱导剂和力学刺激均能促进rBMSCs的骨向分化,且二者之间具有协同作用。Objective To investigate the effect from mechanical stimulation and osteogenic chemical inductor on osteoblastic differentiation markers and formation of calcified nodules in rat bone mesenchymal stem cells（rBMSCs）.Method The rBMSC were cultured in medium contained with or without osteogenic chemical inductor.The cyclic biaxial mechanical strain（2%）,at a frequency of 1 Hz,was applied to the rBMSCs for periods of 2 hours each time,at intervals of 2 hours,3 times every day,lasting 3 days and 6 days,respectively.The mRNA expression of alkaline phosphatase（ALP）,collagen type I（COL I） and osteocalcin（OCN） were analyzed with real time fluorescent quantitation reverse transcription-polymerase chain reaction（qRT-PCR） and formation of calcified nodules were detected with alizarin red staining method.Results The mRNA expression of ALP,COL I and OCN were significantly increased in induced group compared with that in the corresponding uninduced group and calcified nodule was observed in the osteogenic chemical inductor group after 6 days with mechanical stimulation.Conclusions Osteogenic chemical inductor and mechanical stimulation can promote the osteoblastic differentiation of rBMSCs.

关 键 词：力学刺激 双轴力学应变 成骨细胞 化学诱导剂 大鼠骨髓间充质干细胞 成骨分化 

分 类 号：R3[医药卫生—基础医学]