动态增强MRI评价内皮抑素对小鼠结肠癌肿瘤血管生成的研究  

Study of Dynamic Contrast-Enhanced MRI in Evaluating the Angiogenesis of Endostatin in Colorectal Carcinoma

作　　者：杨斌[1] 程鑫[1] 张仕状[1] 王滨[1] 韩明[2] 

机构地区：[1]潍坊医学院附属医院影像中心潍坊医学院医学影像学系,山东潍坊261031 [2]潍坊医学院基础部

出　　处：《实用放射学杂志》2011年第4期620-623,共4页Journal of Practical Radiology

基　　金：基金项目：国家自然科学基金资助项目（30572323,30772716,81072977）;潍坊市科委资助项目（潍科字2010第02045号）.

摘　　要：目的 探讨动态增强MRI评价内皮抑素对小鼠结肠癌肿瘤血管生成的价值.方法 建立小鼠结肠癌皮下移植瘤模型,1周后筛选成瘤均匀的32只随机分成内皮抑素（ES）组和生理盐水（NS）组,分别给予内皮抑素6 mg/（kg＊d）和0.2 ml生理盐水皮下注射,连续注射14 d后行MRI动态增强扫描,观察MRI动态增强时间-信号强度曲线（time-signal intensity curve,TIC）形态,并比较首次增强斜率（Sfirst）的差异及其与MVD、VEGF表达间的相关性.结果 ES组TIC呈缓慢上升型,而NS组呈速升缓降型.NS组的Sfirst为（4.92±1.03） unit/s,ES组为（3.17±0.91） unit/s,NS组的Sfirst明显高于ES组 （P〈0.05）.ES组VEGF表达水平较NS组降低、MVD分布也较稀疏.Sfirst与VEGF及Ⅷ因子的表达均呈明显正相关（P〈0.05）.结论 动态增强MRI能较准确评价内皮抑素对小鼠结肠癌肿瘤血管生成的影响.Objective To explore the value of dynamic contrast enhanced MRI in evaluating the angiogenesis of endostatin in colo rectal carcinoma. Methods The models of colorectal carcinoma subcutaneously transplantation in mice were established, one week later, 32 tumor-bearing mice were sieved out and divided randomly into two groups：group A and group B, treated with intraperito- neal injection of endostatin（ES） 6 mg/（kg . d） and 0.2 ml normal saline（ NS） respectively for 14 days. Subsequently, dynamic con- trast-enhanced MRI was performed and the shapes of time-signal intensity curve （TIC） were observed. The difference of first slope was compared and the relation of the first slope and MVD and VEGF expression between group A and B,respectively. Results The TIC appeared as a slowly ascending type in group A and rapidly ascending-slowly descending one in group B. The first slopes were （4.92±1.03） unit/s and （3.17±0.91） unit/s in group A and B respectively. There was significant difference between these two groups （P〈0.05）. The expressions of VEGF and MVD in group A were lower than which in group B. There was positive correlation between first slope and expressions of VEGF and MVD （P〈0.05）. Conclusion Dynamic contrast-enhanced MRI can evaluate correctly the angiogenesis of endostatin in the models of eolorectal carcinoma in mice.

关 键 词：鼠 磁共振成像 内皮抑素 移植瘤模型 肿瘤血管 

分 类 号：R332[医药卫生—人体生理学] R735.35[医药卫生—基础医学]