6个紫花苜蓿材料的核型及其亲缘关系分析  

Karyotype and Cluster Analysis of 6 Alfalfa Materials

作　　者：张雪婷[1] 杨文雄[1] 杨芳萍[1] 师尚礼[2] 尹国丽[2] 

机构地区：[1]甘肃省农业科学院,兰州730070 [2]甘肃农业大学草业学院,兰州730070

出　　处：《西北植物学报》2011年第4期671-676,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：农业部牧草种质资源保护项目(NB08-2130135-43)

摘　　要：运用染色体压片法对陇东野生紫花苜蓿(Medicago sativaL.)等6个紫花苜蓿材料进行核型分析,并通过核型似近系数对供试材料进行聚类分析。结果显示:(1)全部供试材料皆为2n=32的四倍体,核型进化由高到低依次为:[陇东野生紫花苜蓿、新疆大叶紫花苜蓿(M.sativa,Xinjiangdaye)]2B>[皇后2000紫花苜蓿(M.sativa,Regina 2000)、陇东紫花苜蓿(M.sativa,Longdong)、Pick 8925紫花苜蓿(M.sativa,Pick 8925)]1B>[肇东紫花苜蓿(M.sativa,Zhaodong)]1A。(2)供试材料间的核型似近系数(λ)均在0.900 0以上,并在进化距离(De)0.073 8处分为两类,陇东野生紫花苜蓿和新疆大叶紫花苜蓿归为一类,其它供试材料归为另一类。研究表明,陇东野生紫花苜蓿的染色体较不对称,与其它供试材料相比,亲缘关系远,系统演化快。Karyotype analysis and cluster analysis was studied on 6 alfalfa materials by chromosome flaking and karyotype resemblance-near coefficient.The result showed that each material was tetraploid with 32 chromosomes.The level of all materials on karyotype evolution was（Medicago sativa L.,M.sativa,Xinjiangdaye） 2B（M.sativa,Regina 2000,M.sativa,Longdong,M.sativa,Pick 8925）1B（M.sativa,Zhaodong） 1A.However,karyotype resemblance-near coefficient of each material was above 0.900 0.Furthermore,all materials were divided into 2 categories when the value of evolutionary distance was 0.073 8,which M.sativa L.and M.sativa,Xinjiangdaye belonging to one category and the rest ones belonging to another category.All above showed the shape of chromosomes of M.sativa L.was more asymmetry.And the speed of its system evolution was faster than any other material in this experiment.

关 键 词：陇东野生紫花苜蓿 核型分析 聚类分析 进化 

分 类 号：Q343.22[生物学—遗传学]