芒果子叶切段不定根形成的影响因素分析  

Factors of Influencing Adventitious Root Formation of Cotyledon Segments in Mango

作　　者：李运合[1] 马杰[2] 吴永杰[3] 孙光明[1] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [2]河南科技学院,河南新乡453003 [3]河北农林科学院昌黎果树研究所,河北昌黎066600

出　　处：《西北植物学报》2011年第4期842-847,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30470110);中国热带农业科学院南亚所中央级公益性科研院所基本科研业务费专项(sscri201014)

摘　　要：分别用不同成熟时间、不同取材部位、不同品种、不同大小的芒果子叶切段为外植体进行不定根的诱导,以探讨影响芒果子叶切段不定根形成能力的原因。结果表明,芒果子叶切段的生根能力随着芒果成熟度的增加而逐渐提高,花后50和60 d的2.0 cm长子叶切段都无不定根形成,从花后70 d开始有不定根形成,此时生根率为28.6%,之后其生根能力迅速提高,在花后90 d生根率达到76.7%,之后生根率稳定保持直到110 d果实成熟。成熟芒果的子叶切段长度(2.0、1.0、0.5和0.2 cm)对不定根的形成有显著影响,0.2 cm的子叶切段上无不定根形成,另外3个长度的切段都有不定根形成,且生根能力随着长度的增大而逐渐提高。取材位置(靠近或远离胚轴)则对生根影响不大,且紫花芒、红芒、青皮芒等几种常见芒果品种的不定根形成能力基本相同。In order to research the factors of influencing adventitious rooting from cut surface of mango cotyledon segments,the segments＇ rooting abilities of different collect time,different lengths,different cut positions and different varieties were examined.The results showed that rooting ability of cotyledon segments increased with the increase of maturity of fruits,there was no root formation at the 2 cm cotyledon segments from the embryos of 50 and 60 days after flowering.At 70 days after flowering,there was adventitious root formed on PCS（proximal cut surface）,the percentage rooting was 28.6%;After that,the rooting ability was increased sharply and reached to 76.7% at 90 days after flowering,but then kept at a steady level until matured at 110 days after flowering.The length（2.0,1.0,0.5 and 0.2 cm） of cotyledon segment from maturated fruits was closely related with the rooting ability：there was no root formed on 0.2 cm segment,whereas roots formed on the other three length segments and the rooting ability increased with the increase of lengths.Compared the rooting abilities of Mangifera indica L.var.Zihua,M.indica L.var.Okrong and Mangifera indica L.var.Zill,all of them were almost same.Cut position of cotyledon segment（i.e.,close to the proximal or not） has little effect on the rooting.

关 键 词：芒果 子叶切段 不定根 生根能力 生根体系 

分 类 号：Q813.13[生物学—生物工程]