外源NO对干旱胁迫下平邑甜茶幼苗叶绿素荧光参数和光合速率的影响  

Effects of Exogenous Nitric Oxide on Chlorophyll Fluorescence Parameters and Photosynthesis Rate in Malus hupehensis Seedlings Under Water Stress

作　　者：曹慧[1,2] 王孝威[1] 邹岩梅 束怀瑞 

机构地区：[1]潍坊学院生物工程学院,山东潍坊261061 [2]国家苹果工程技术研究中心,山东泰安271018

出　　处：《园艺学报》2011年第4期613-620,共8页Acta Horticulturae Sinica

基　　金：国家现代苹果产业技术体系项目;潍坊市科技发展计划项目(2009038);潍坊学院优秀学术团队项目(2008Z01)

摘　　要：以20%聚乙二醇（PEG6000）处理模拟干旱,利用一氧化氮（nitric oxide,NO）供体硝普钠（sodium nitroprusside,SNP）处理平邑甜茶[Malus hupehensis（Pamp.）Rehd.]幼苗,探讨外源NO对水分胁迫下平邑甜茶幼苗叶绿素荧光参数和光合速率的影响。结果表明：水分胁迫下平邑甜茶幼苗Fo显著上升,Fv/Fm、qP、ETR、Yield、叶绿素含量和光合速率显著降低,qN在胁迫的前3d呈上升趋势,之后大幅度下降;通过外施不同浓度的SNP（100－700μmol·L-1）均使水分胁迫下平邑甜茶幼苗Fo上升及Fv/Fm、ETR、qP、Yield、叶绿素含量和光合速率下降幅度明显减小。不同处理缓解作用为：300μmol·L-1SNP〉500μmol·L-1SNP〉100μmol·L-1SNP〉700μmol·L-1SNP,其中300－500μmol·L-1SNP处理缓解效应明显,但以300μmol·L-1SNP处理效果最好。The effects of exogenous NO on chlorophyll fluorescence parameters and photosynthesis rate in Malus hupehensis（Pamp.）Rehd.seedlings were studied in simulating drought condition of nutrient solution cultivation containing 20% polyethylene glycol 6000（PEG6000）and sodium nitroprusside（SNP）.The results showed that the Fo significantly increased,however,the Fv/Fm,qP,ETR,Yield,chlorophyll contents and photosynthesis rate all markedly decreased when the seedlings treated with PEG6000.Meanwhile,the qN increased within 3 days and then drastically decreased.On the other hand,the increase of Fo and the decrease of Fv/Fm,qP,ETR,Yield,chlorophyll contents and photosynthesis rate were all observed when the seedlings treated with 100-700 μmol · L-1 SNP.The results also indicated that exogenous nitric oxide with different treatments all had alleviated effects on Malus hupehensis（Pamp.） Rehd.seedlings and the alleviation function in the treatments was 300 μmol · L-1 SNP 500 μmol · L-1 SNP 100 μmol · L-1 SNP〉 700 μmol · L-1 SNP.Moreover,there were remarkably alleviated effects with treatments from 300 μmol · L-1 SNP to 500 μmol · L-1 SNP,in which 300 μmol · L-1 SNP showed the best effect.

关 键 词：平邑甜茶 干旱胁迫 一氧化氮 叶绿素荧光参数 光合速率 

分 类 号：S661[农业科学—果树学]