草莓果实发育过程中糖代谢相关基因的表达分析  

Transcriptional Analysis of Sugar Metabolism-related Genes During Strawberry Fruit Development

作　　者：柴叶茂[1] 贾海锋[1] 李春丽[1] 秦岭[1] 沈元月[1] 

机构地区：[1]农业应用新技术北京市重点实验室,北京农学院植物科学技术学院,北京102206

出　　处：《园艺学报》2011年第4期637-643,共7页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30971977);北京市自然科学基金项目(6082005);北京市自然科学基金;北京市教委联合资助重点项目(KZ200910020001)

摘　　要：为了分析草莓(Fragaria×ananassa Duch.)果实发育过程中可溶性糖积累与相关基因表达量的关系,以'杜克拉'草莓7个发育时期的果实为试材,利用实时荧光定量RT-PCR的方法,分析果实发育中蔗糖转运蛋白及糖代谢关键酶(酸性转化酶、蔗糖合成酶和蔗糖磷酸合成酶)4个基因的表达量以及可溶性糖含量的变化趋势。结果表明,蔗糖、葡萄糖和果糖含量随着果实发育都呈逐渐增加的趋势,尤其蔗糖积累与果实成熟的关系较密切。随着果实发育,蔗糖磷酸合成酶基因表达量呈逐渐增加的趋势,尤其在果实发育的后期增加较快;蔗糖合成酶和酸性转化酶基因表达量除了白熟期和转色期外,呈逐渐降低的趋势;蔗糖转运蛋白基因表达量呈先升后降,降后又升的变化趋势,尤其在白熟期前急剧增加。结合可溶性糖和基因变化趋势分析表明,蔗糖转运蛋白和蔗糖磷酸合成酶在草莓果实发育过程中发挥着重要的作用。To analyze the relationship of sugar accumulation with sugar-related gene expression during strawberryfi＇uit development, receptacle of ＇Fugilia＇ strawberry （Fragaria × ananassaDuch.） was used and real-time RT-PCR primers were designed to analyze both changes of the mRNA expression of four sugar accumulation-related genes and soluble sugar contents, respectively. The main results showed that sucrose, glucose and fructose contents increased continually during fruit development, in particular sucrose accumulation was closely related to fruit ripening. The mRNA expression level of sucrose phosphate Synthase（ SPS）gene increased gradually during fruit development, and a rapid increase occurred in later developmental stages; The mRNA expression level of sucrose synthase （SS） and acid invertase （AI） decreased continually in addition to white or turning stages; The mRNA expression level of sucrose transporter （SUT1） displayed increased firstly, and then declined, finally increased again, in particular increased rapidly before white period. Combinational analysis of the changes of soluble sugars content and gene expression demonstrated that sucrose carrier and sucrose phosphate synthase play an important role in regulation of strawberry fruit ripening.

关 键 词：草莓 果实 发育 可溶性糖 蔗糖转运蛋白 荧光定量PCR 

分 类 号：S663.1[农业科学—果树学]